AP02198SU-N

antibody from OriGene

Targeting: PTMA TMSA

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP02198SU-N

Provider

OriGene

Product name

prothymosin, alpha (PTMA) (75-109) rabbit polyclonal antibody, Serum

Antibody type

Polyclonal

Description

prothymosin, alpha (PTMA) (75-109) rabbit polyclonal antibody, Serum

Host

Rabbit

Conjugate

Unconjugated

Epitope

PTMA

Antibody clone number

NULL

Vial size

100 µl

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Figure 1. Western Blot analysis of AP02198SU specificity. Extracts of 231 and MCF-7 cells were separated by SDS-PAGE and immunoblotted with AP02198SU (2 ug/ml). AP02198SU Detects only Prothymosin alpha in cells extracts. Martini PG et al. (2000) Mol Cell Biol 20(17):6224-32.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Figure 2. Immunohistochemistry of Prothymosin alpha staining in Paraffin sections of Rat testis. The sections were incubated with AP02198SU (1/1000)and detected using ABC method. The sections were counterstained with hematoxylin. Image A: AP02198SU stains Lydig cells (arrowhead), pachytene primary spermatozoides (arrow), and newly formed spermatids (asterisks). Image B: Control section without primary antibody. Scale Bar = 20 ?m. Ferrara D et al. (2010) J. Cell. Physiol. 224(2):362-8.

Validation comment

IHC