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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX632126 - Provider product page
- Provider
- GeneTex
- Product name
- TEF1 antibody [GT13112]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- TEF1 antibody [GT13112] detects TEF1 protein by western blot analysis. whole cell extracts and extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with TEF1 antibody [GT13112] (GTX632126) at a dilution of 1:500.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TEF1 antibody [GT13112] detects TEF1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TEF1 protein stained by TEF1 antibody [GT13112] (GTX632126) diluted at 1:400.Red: Phalloidin, a F-actin marker, diluted at 1:200.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of TEF1 protein from HeLa nuclear extracts using 5 £gg of TEF1 antibody [GT13112] (GTX632126).Western blot analysis was performed using TEF1 antibody [GT13112] (GTX632126).EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
