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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- MA5-27786 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TEF1 Monoclonal Antibody (GT13112)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: HeLa NE
- Antibody clone number
- GT13112
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TEF1 in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with TEF1 monoclonal antibody (Product # MA5-27786) using a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TEF1 Monoclonal Antibody (GT13112) (Product # MA5-27786). HeLa whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TEF1 Monoclonal Antibody (GT13112) (Product # MA5-27786) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using TEF1 Monoclonal Antibody (GT13112) (Product # MA5-27786) and a~48 kDa band corresponding to TEAD1 was observed across all the cell lines tested along with an uncharacterized band (*) at 30 kDa. Whole cell extracts (30 µg lysate) of A431 (Lane 1), HeLa (Lane 2), U2OS (Lane 3), BeWo (Lane 4), HEL 92.1.7 (Lane 5), U937 (Lane 6) and MOLT4 (Lane 7) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TEF1 Monoclonal Antibody (GT13112) detects TEF1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TEF1 protein stained by TEF1 Monoclonal Antibody (GT13112) (Product # MA5-27786) diluted at 1:400. Red: Phalloidin, a F-actin marker, diluted at 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of TEF1 was performed in HeLa nuclear extracts using 5 µg of TEF1 Monoclonal Antibody (GT13112) (Product # MA5-27786). Samples were transferred to a membrane and probed with TEF1 Monoclonal Antibody (GT13112) as a primary antibody and an HRP-conjugated anti-Mouse IgG was used as a secondary antibody.
