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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- NBP2-20076 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal RAP30 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RAP30 Antibody [NBP2-20076] - Sample (30 ug of whole cell lysate) A: NIH-3T3 7. 5% SDS PAGE gel, diluted at 1:3000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RAP30 Antibody [NBP2-20076] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7. 5% SDS PAGE gel, diluted at 1:3000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RAP30 Antibody [NBP2-20076] - 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of TFIIF protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of TFIIF by 2.5 ug of TFIIF antibody 7.5% SDS-PAGE The immunoprecipitated TFIIF protein was detected by TFIIF antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation (ChIP): RAP30 Antibody [NBP2-20076] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-TFIIF antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.
