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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-30050 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GTF2F1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, NIH-3T3. Predicted reactivity: Mouse (87%), Rat (87%), Rhesus Monkey (98%), Chimpanzee (97%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Exon-Mediated Activation of Transcription Starts.
Fiszbein A, Krick KS, Begg BE, Burge CB
Cell 2019 Dec 12;179(7):1551-1565.e17
Cell 2019 Dec 12;179(7):1551-1565.e17
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GTF2F1 Polyclonal Antibody (Product # PA5-30050). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3 . 7.5% SDS PAGE. GTF2F1 Polyclonal Antibody (Product # PA5-30050) diluted at 1:3,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GTF2F1 Polyclonal Antibody (Product # PA5-30050). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. Lane C: HeLa . 7.5% SDS PAGE. GTF2F1 Polyclonal Antibody (Product # PA5-30050) diluted at 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or GTF2F1 Polyclonal Antibody (Product # PA5-30050). The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GTF2F1 Polyclonal Antibody immunoprecipitates TFIIF protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 µg whole cell lysate/extract of TFIIF protein expressing 293T cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of TFIIF by 2.5 µg of GTF2F1 Polyclonal Antibody (Product # PA5-30050) 7.5% SDS-PAGE The immunoprecipitated TFIIF protein was detected by GTF2F1 Polyclonal Antibody (Product # PA5-30050) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.
