A300-840A

antibody from Invitrogen Antibodies

Targeting: PPP4R3A FLFL1, FLJ20707, KIAA2010, MSTP033, PP4R3, SMEK1, smk-1, smk1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-840A

Provider

Invitrogen Antibodies

Product name

PPP4R3 Alpha Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Concentration

0.20 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PPP4R3 Alpha by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg) and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PPP4R3 alpha antibody BL3121 (Cat. No. A300-840A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). PPP4R3 alpha was also immunoprecipitated by rabbit anti-PPP4R3 alpha antibody BL3120 (Cat. No. A300-839A), which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 0.5 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PPP4R3 Alpha by immunohistochemistry. Sample: FFPE sections of human colon carcinoma (left) and breast carcinoma (right). Antibody: Affinity purified rabbit anti- PPP4R3 Alpha (Cat. No. A300-840A Lot1) used at a dilution of 1:200 (1µg/ml) and 1:80 (2.5 µg/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.