17114-1-AP

antibody from Invitrogen Antibodies

Targeting: UPF3A HUPF3A, RENT3A, UPF3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

17114-1-AP

Provider

Invitrogen Antibodies

Product name

UPF3A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: AHDYFEFFA ADLSLYPHLY SRAYINFRNP DDILLFRDRF DGYIFLDSKD PEYKKFLETY CVEEEKTSAN PETLLGEMEA KTRELIARRT TPLLEYIKNR KLEKQRIREE KREERRRREL EKKRLREEEK RRRREEERCK KKETDKQKKI AEKEVRIKLL KKPEKGEEPT TEKPKERGEE IDTGGGKQES CAPGAVVKAR PMEGSLEEPQ ETSHSGSDKE HRDVERSQEQ ESEAQRYHVD DGRRHRAHHE PERLSRRSED EQRWGKGPGQ DRGKKGSQDS GAPGEAMERL GRAQRCDDSP APRKERLANK DRPALQLYDP GARFRARECG GNRRICKAEG SGTGPEKREE AE (93-443 aa encoded by BC023569)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.26 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 17114-1-AP (UPF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human testis tissue were subjected to SDS PAGE followed by western blot with 17114-1-AP (UPF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 17114-1-AP ( UPF3A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 17114-1-AP (UPF3A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 17114-1-AP (UPF3A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 17114-1-AP (UPF3A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart using 17114-1-AP (UPF3A antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart using 17114-1-AP (UPF3A antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 17114-1-AP ( UPF3A Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 17114-1-AP ( UPF3A Antibody) at dilution of 1:200 (under 40x lens).