Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP1-89976 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89976, RRID:AB_11011044
- Product name
- Rabbit Polyclonal Transcription factor E3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Transcription factor E3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Hypoxia induces TFE3 expression in head and neck squamous cell carcinoma.
Resetting transcription factor control circuitry toward ground-state pluripotency in human.
Derivation of novel human ground state naive pluripotent stem cells.
Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3.
Inactivation of the FLCN tumor suppressor gene induces TFE3 transcriptional activity by increasing its nuclear localization.
Sun ZJ, Yu GT, Huang CF, Bu LL, Liu JF, Ma SR, Zhang WF, Liu B, Zhang L
Oncotarget 2016 Mar 8;7(10):11651-63
Oncotarget 2016 Mar 8;7(10):11651-63
Resetting transcription factor control circuitry toward ground-state pluripotency in human.
Takashima Y, Guo G, Loos R, Nichols J, Ficz G, Krueger F, Oxley D, Santos F, Clarke J, Mansfield W, Reik W, Bertone P, Smith A
Cell 2014 Sep 11;158(6):1254-1269
Cell 2014 Sep 11;158(6):1254-1269
Derivation of novel human ground state naive pluripotent stem cells.
Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D, Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, Novershtern N, Hanna JH
Nature 2013 Dec 12;504(7479):282-6
Nature 2013 Dec 12;504(7479):282-6
Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3.
Betschinger J, Nichols J, Dietmann S, Corrin PD, Paddison PJ, Smith A
Cell 2013 Apr 11;153(2):335-47
Cell 2013 Apr 11;153(2):335-47
Inactivation of the FLCN tumor suppressor gene induces TFE3 transcriptional activity by increasing its nuclear localization.
Hong SB, Oh H, Valera VA, Baba M, Schmidt LS, Linehan WM
PloS one 2010 Dec 29;5(12):e15793
PloS one 2010 Dec 29;5(12):e15793
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Transcription factor E3 Antibody [NBP1-89976] - Simple Western lane view shows a specific band for TFE3 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Transcription factor E3 Antibody [NBP1-89976] - Electropherogram image(s) of corresponding Simple Western lane view. Transcription factor E3 antibody was used at 1:30 dilution on RT-4 lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Transcription factor E3 Antibody [NBP1-89976] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Transcription factor E3 Antibody [NBP1-89976] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TFE3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Transcription factor E3 Antibody [NBP1-89976] - Western blot analysis of TFE3 in total lysates of MIN6 mouse insulinoma while cell lysates. Customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Transcription factor E3 Antibody [NBP1-89976] - Staining of human testis shows moderate nuclear positivity in a subset of cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Transcription factor E3 Antibody [NBP1-89976] - Staining of human kidney shows moderate nuclear positivity in renal glomeruli and tubuli cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Transcription factor E3 Antibody [NBP1-89976] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Transcription factor E3 Antibody [NBP1-89976] - Staining of human prostate shows moderate to strong nuclear positivity in glandular cells.