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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB5580 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse F4/80 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse F4/80/EMR1.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q61549- Isotype
- IgG
- Antibody clone number
- 521204
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Mannan-binding lectin deficiency exacerbates sterile liver injury in mice through enhancing hepatic neutrophil recruitment.
Osteopontin in the host response to Leishmania amazonensis.
Self-implantable double-layered micro-drug-reservoirs for efficient and controlled ocular drug delivery.
Depletion of Nsd2-mediated histone H3K36 methylation impairs adipose tissue development and function.
Central role of myeloid MCPIP1 in protecting against LPS-induced inflammation and lung injury.
Combination of Fluorescent in situ Hybridization (FISH) and Immunofluorescence Imaging for Detection of Cytokine Expression in Microglia/Macrophage Cells.
MerTK cleavage limits proresolving mediator biosynthesis and exacerbates tissue inflammation.
Macrophages as IL-25/IL-33-responsive cells play an important role in the induction of type 2 immunity.
CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation.
Extracellular adenosine signaling induces CX3CL1 expression in the brain to promote experimental autoimmune encephalomyelitis.
Zhou J, Li J, Yu Y, Liu Y, Li H, Liu Y, Wang J, Zhang L, Lu X, Chen Z, Zuo D
Journal of leukocyte biology 2019 Jan;105(1):177-186
Journal of leukocyte biology 2019 Jan;105(1):177-186
Osteopontin in the host response to Leishmania amazonensis.
Giraud E, Rouault E, Fiette L, Colle JH, Smirlis D, Melanitou E
BMC microbiology 2019 Feb 8;19(1):32
BMC microbiology 2019 Feb 8;19(1):32
Self-implantable double-layered micro-drug-reservoirs for efficient and controlled ocular drug delivery.
Than A, Liu C, Chang H, Duong PK, Cheung CMG, Xu C, Wang X, Chen P
Nature communications 2018 Nov 6;9(1):4433
Nature communications 2018 Nov 6;9(1):4433
Depletion of Nsd2-mediated histone H3K36 methylation impairs adipose tissue development and function.
Zhuang L, Jang Y, Park YK, Lee JE, Jain S, Froimchuk E, Broun A, Liu C, Gavrilova O, Ge K
Nature communications 2018 May 4;9(1):1796
Nature communications 2018 May 4;9(1):1796
Central role of myeloid MCPIP1 in protecting against LPS-induced inflammation and lung injury.
Li Y, Huang X, Huang S, He H, Lei T, Saaoud F, Yu XQ, Melnick A, Kumar A, Papasian CJ, Fan D, Fu M
Signal transduction and targeted therapy 2017;2:17066
Signal transduction and targeted therapy 2017;2:17066
Combination of Fluorescent in situ Hybridization (FISH) and Immunofluorescence Imaging for Detection of Cytokine Expression in Microglia/Macrophage Cells.
Fe Lanfranco M, Loane DJ, Mocchetti I, Burns MP, Villapol S
Bio-protocol 2017 Nov 20;7(22)
Bio-protocol 2017 Nov 20;7(22)
MerTK cleavage limits proresolving mediator biosynthesis and exacerbates tissue inflammation.
Cai B, Thorp EB, Doran AC, Subramanian M, Sansbury BE, Lin CS, Spite M, Fredman G, Tabas I
Proceedings of the National Academy of Sciences of the United States of America 2016 Jun 7;113(23):6526-31
Proceedings of the National Academy of Sciences of the United States of America 2016 Jun 7;113(23):6526-31
Macrophages as IL-25/IL-33-responsive cells play an important role in the induction of type 2 immunity.
Yang Z, Grinchuk V, Urban JF Jr, Bohl J, Sun R, Notari L, Yan S, Ramalingam T, Keegan AD, Wynn TA, Shea-Donohue T, Zhao A
PloS one 2013;8(3):e59441
PloS one 2013;8(3):e59441
CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation.
Denoyer A, Godefroy D, Célérier I, Frugier J, Riancho L, Baudouin F, Rostène W, Baudouin C
Mucosal immunology 2012 Nov;5(6):702-11
Mucosal immunology 2012 Nov;5(6):702-11
Extracellular adenosine signaling induces CX3CL1 expression in the brain to promote experimental autoimmune encephalomyelitis.
Mills JH, Alabanza LM, Mahamed DA, Bynoe MS
Journal of neuroinflammation 2012 Aug 10;9:193
Journal of neuroinflammation 2012 Aug 10;9:193
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- F4/80 in Mouse Lung. F4/80 was detected in immersion fixed frozen sections of mouse lung using Rat Anti-Mouse F4/80 Monoclonal Antibody (Catalog # MAB5580) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in macrophages. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of F4/80/EMR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 Monoclonal Antibody (Catalog # MAB5580, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113).
