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LearnBSM-54170R
antibody from Invitrogen Antibodies
Targeting: PSMA1
HC2, MGC14542, MGC14575, MGC14751, MGC1667, MGC21459, MGC22853, MGC23915, NU, PROS30
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- BSM-54170R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSMA1 Monoclonal Antibody (10A2)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 10A2
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lane 1: Jurkat Cells; Lane 2: Hela Cells; Lane 3: HepG2 Cells; Lane 4: 293 Cells; Probed with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lane 1: PC-12 Cells; Lane 2: Mouse Spleen; Lane 3: Rat Spleen; Probed with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF(ICC) staining with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF(ICC) staining with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF(ICC) staining with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:300 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Mouse Cerebellum tissue incubated with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) 1:100 dilution (red) compared with an unlabeled control (cells with out incubation with primary antibody; black).
