Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- H00001392-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001392-M02, RRID:AB_877564
- Product name
- Mouse Monoclonal Corticotropin Releasing Factor Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CRH (2B11)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Negative effects of progesterone receptor isoform-A on human placental activity of the noncanonical NF-κB signaling.
Glucocorticoid receptor signaling contributes to constitutive activation of the noncanonical NF-κB pathway in term human placenta.
RelB/NF-κB2 regulates corticotropin-releasing hormone in the human placenta.
Wang B, Parobchak N, Rosen M, Roche N, Rosen T
The Journal of clinical endocrinology and metabolism 2014 Feb;99(2):E320-8
The Journal of clinical endocrinology and metabolism 2014 Feb;99(2):E320-8
Glucocorticoid receptor signaling contributes to constitutive activation of the noncanonical NF-κB pathway in term human placenta.
Wang B, Palomares K, Parobchak N, Cece J, Rosen M, Nguyen A, Rosen T
Molecular endocrinology (Baltimore, Md.) 2013 Feb;27(2):203-11
Molecular endocrinology (Baltimore, Md.) 2013 Feb;27(2):203-11
RelB/NF-κB2 regulates corticotropin-releasing hormone in the human placenta.
Wang B, Parobchak N, Rosen T
Molecular endocrinology (Baltimore, Md.) 2012 Aug;26(8):1356-69
Molecular endocrinology (Baltimore, Md.) 2012 Aug;26(8):1356-69
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Corticotropin Releasing Factor Antibody (2B11) [H00001392-M02] - Analysis of CRH expression in transfected 293T cell line by CRH monoclonal antibody (M02), clone 2B11.Lane 1: CRH transfected lysate(21.4 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Corticotropin Releasing Factor Antibody (2B11) [H00001392-M02] - Detection limit for recombinant GST tagged CRH is 0.3 ng/ml as a capture antibody.