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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunohistochemistry [2]
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- Product number
- PA1-37499 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CRF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with chicken, canine, amphibian, mouse, porcine and rat based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is brain or placenta tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 1 mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Acupuncture Alleviates Anxiety and 22-kHz Ultrasonic Vocalizations in Rats Subjected to Repeated Alcohol Administration by Modulating the Brain-Derived Neurotrophic Factor/Corticotropin-Releasing Hormone Signaling Pathway.
Fibroblast growth factor 8 regulates postnatal development of paraventricular nucleus neuroendocrine cells.
Seo SY, Bang SK, Kang SY, Cho SJ, Choi KH, Ryu YH
International journal of molecular sciences 2021 Apr 14;22(8)
International journal of molecular sciences 2021 Apr 14;22(8)
Fibroblast growth factor 8 regulates postnatal development of paraventricular nucleus neuroendocrine cells.
Rodriguez KM, Stevenson EL, Stewart CE, Linscott ML, Chung WC
Behavioral and brain functions : BBF 2015 Nov 4;11(1):34
Behavioral and brain functions : BBF 2015 Nov 4;11(1):34
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Corticotropin Releasing Factor using anti-Corticotropin Releasing Factor Polyclonal Antibody (Product # PA5-32364) in Brain Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CRF using a polyclonal antibody (Product # PA1-37499).
