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Learn16145-1-AP
antibody from Invitrogen Antibodies
Targeting: POLR1E
FLJ13390, FLJ13970, PAF53, PRAF1, RPA49
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- 16145-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLR1E Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: AELFNMQPL FSDVSVESEL ALESQTKTYR EKMDSCIEAF GTTKQKRALN TRRMNRVGNE SLNRAVAKAA ETIIDTKGVT ALVSDAIHND LQDDSLYLPP CYDDAAKPED VYKFEDLLSP AEYEALQSPS EAFRNVTSEE ILKMIEENSH CTFVIEALKS LPSDVESRDR QARCIWFLDT LIKFRAHRVV KRKSALGPGV PHIINTKLLK HFTCLTYNNG RLRNLISDSM KAKITAYVII LALHIHDFQI DLTVLQRDLK LSEKRMMEIA KAMRLKISKR KVSVAAGSEE DHKLGTLSLP LPPAQTSDRL AKRRKIT (104-419 aa encoded by BC014331 )
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.23 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 16145-1-AP (POLR1E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 16145-1-AP (POLR1E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 16145-1-AP ( POLR1E Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-POLR1E (IP:16145-1-AP, 3ug; Detection:16145-1-AP 1:500) with Jurkat cells lysate 1000ug.
