PA5-116551

antibody from Invitrogen Antibodies

Targeting: POLR1E FLJ13390, FLJ13970, PAF53, PRAF1, RPA49

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-116551

Provider

Invitrogen Antibodies

Product name

POLR1E Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Samples: HeLa, A-549, 293T, Jurkat

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.46 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of POLR1E using POLR1E Polyclonal Antibody (Product # PA5-116551) at a 1:3,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of POLR1E in 200 µg extracts of Jurkat cells using POLR1E Polyclonal Antibody (Product # PA5-116551). Western blot was performed from the immunoprecipitate using the same antibody at a dilution of 1:100