Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C153821 - Provider product page
- Provider
- LSBio
- Product name
- IL6 / Interleukin 6 Antibody (clone 33A12.G9) LS-C153821
- Antibody type
- Monoclonal
- Description
- Antiserum
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 33A12.G9
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot showing detection of Human IL-6. 100 ng of Human IL-6 was run on a 4-20% gel and transferred to 0.45 m nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20C, Anti-Human IL-6 (MOUSE) Antibody (p/n Anti-Human IL-6 (MOUSE) Monoclonal Antibody - 209-301-310) was used at 1:1000 in 1% BSA-TTBS over night at 4?. Peroxidase conjugated Rabbit Anti-mouse secondary antibody was used in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) at 1:40000 for 30 min at 20C and imaged using the Bio-Rad VersaDoc 4000 MP. Band indicates correct 17 kDa molecular weight position expected for Human IL-6.