Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- LS-C154079 - Provider product page
- Provider
- LSBio
- Product name
- IL6 / Interleukin 6 Antibody LS-C154079
- Antibody type
- Polyclonal
- Description
- Antiserum
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-Human IL6 Antibody - Western Blot. Western blot of anti-Human IL6 antibody shows detection of a band ~24 kD in size corresponding to recombinant human IL6 (arrowhead). Molecular weight markers are also shown. After transfer, the membrane was blocked overnight with 1% BSA in TTBS followed by reaction with primary antibody at a 1:500 dilution. Detection occurred using HRP conjugated anti-Rabbit IgG (LS-C60865) secondary antibody diluted 1:40000 in blocking buffer (p/n MB-070) and FemtoMax Chemiluminescent substrate (p/n FEMTOMAX-110). Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).