Antibody data
- Antibody Data
- Antigen structure
- References [24]
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- Product number
- M621B - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-6 Monoclonal Antibody (7IL6), Biotin
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- M621B targets IL-6 in ELISA and WB applications and shows reactivity with Human samples. The M621B immunogen is recombinant human IL-6. M621B detects IL-6 which has a predicted molecular weight of approximately 22 kDa. The M621B IL6 antibody (biotinylated conjugate of clone 7IL6) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M620 (clone 5IL6). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection. Biotinylated antibody M621B (clone 7IL6) and antibody M620 (clone 5IL6) have successfully been used in combination with recombinant IL6 protein SIL6 in ELISA applications.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- 7IL6
- Vial size
- 250 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references Therapeutic effect of Resveratrol in the treatment of osteoarthritis via the MALAT1/miR-9/NF-κB signaling pathway.
Toll-Interleukin 1 Receptor Domain-Containing Adaptor Protein 180L Single-Nucleotide Polymorphism Is Associated With Susceptibility to Recurrent Pneumococcal Lower Respiratory Tract Infections in Children.
Kaposi's sarcoma-associated herpesvirus kaposin B induces unique monophosphorylation of STAT3 at serine 727 and MK2-mediated inactivation of the STAT3 transcriptional repressor TRIM28.
Th1 responses to beta-amyloid in young humans convert to regulatory IL-10 responses in Down syndrome and Alzheimer's disease.
Inhibitors of p38 suppress cytokine production in rheumatoid arthritis synovial membranes: does variable inhibition of interleukin-6 production limit effectiveness in vivo?
Development of an 8-plex Luminex assay to detect swine cytokines for vaccine development: assessment of immunity after porcine reproductive and respiratory syndrome virus (PRRSV) vaccination.
A component of polysaccharide peptidoglycan complex on Lactobacillus induced an improvement of murine model of inflammatory bowel disease and colitis-associated cancer.
Human mast cell activation with virus-associated stimuli leads to the selective chemotaxis of natural killer cells by a CXCL8-dependent mechanism.
IgE modulates neutrophil survival in asthma: role of mitochondrial pathway.
IgE modulates neutrophil survival in asthma: role of mitochondrial pathway.
Protein microarray platform for the multiplex analysis of biomarkers in human sera.
Probiotic Lactobacillus-induced improvement in murine chronic inflammatory bowel disease is associated with the down-regulation of pro-inflammatory cytokines in lamina propria mononuclear cells.
Tumor-infiltrating lymphocyte secretion of IL-6 antagonizes tumor-derived TGF-beta 1 and restores the lymphokine-activated killing activity.
Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis.
Interleukin-6 fused to an anti-idiotype antibody in a vaccine increases the specific humoral immune response against CA125+ (MUC-16) ovarian cancer.
A nonsteroidal glucocorticoid receptor antagonist.
Serum cytokine levels, cigarette smoking and airway responsiveness among pregnant women.
Ambient air particulates stimulate alveolar macrophages of smokers to promote differentiation of myeloid precursor cells.
Ambient air particulates stimulate alveolar macrophages of smokers to promote differentiation of myeloid precursor cells.
Release of vasoactive cytokines by antibody-enhanced dengue virus infection of a human mast cell/basophil line.
Release of vasoactive cytokines by antibody-enhanced dengue virus infection of a human mast cell/basophil line.
Regulation of interleukin-12 by complement receptor 3 signaling.
Regulation of interleukin-12 by complement receptor 3 signaling.
Sustained high levels of circulating chaperoned interleukin-6 after active specific cancer immunotherapy.
Zhang G, Zhang H, You W, Tang X, Li X, Gong Z
Experimental and therapeutic medicine 2020 Mar;19(3):2343-2352
Experimental and therapeutic medicine 2020 Mar;19(3):2343-2352
Toll-Interleukin 1 Receptor Domain-Containing Adaptor Protein 180L Single-Nucleotide Polymorphism Is Associated With Susceptibility to Recurrent Pneumococcal Lower Respiratory Tract Infections in Children.
Siebert JN, Hamann L, Verolet CM, Gameiro C, Grillet S, Siegrist CA, Posfay-Barbe KM
Frontiers in immunology 2018;9:1780
Frontiers in immunology 2018;9:1780
Kaposi's sarcoma-associated herpesvirus kaposin B induces unique monophosphorylation of STAT3 at serine 727 and MK2-mediated inactivation of the STAT3 transcriptional repressor TRIM28.
King CA
Journal of virology 2013 Aug;87(15):8779-91
Journal of virology 2013 Aug;87(15):8779-91
Th1 responses to beta-amyloid in young humans convert to regulatory IL-10 responses in Down syndrome and Alzheimer's disease.
Loewenbrueck KF, Tigno-Aranjuez JT, Boehm BO, Lehmann PV, Tary-Lehmann M
Neurobiology of aging 2010 Oct;31(10):1732-42
Neurobiology of aging 2010 Oct;31(10):1732-42
Inhibitors of p38 suppress cytokine production in rheumatoid arthritis synovial membranes: does variable inhibition of interleukin-6 production limit effectiveness in vivo?
Page TH, Brown A, Timms EM, Foxwell BM, Ray KP
Arthritis and rheumatism 2010 Nov;62(11):3221-31
Arthritis and rheumatism 2010 Nov;62(11):3221-31
Development of an 8-plex Luminex assay to detect swine cytokines for vaccine development: assessment of immunity after porcine reproductive and respiratory syndrome virus (PRRSV) vaccination.
Lawson S, Lunney J, Zuckermann F, Osorio F, Nelson E, Welbon C, Clement T, Fang Y, Wong S, Kulas K, Christopher-Hennings J
Vaccine 2010 Jul 19;28(32):5356-64
Vaccine 2010 Jul 19;28(32):5356-64
A component of polysaccharide peptidoglycan complex on Lactobacillus induced an improvement of murine model of inflammatory bowel disease and colitis-associated cancer.
Matsumoto S, Hara T, Nagaoka M, Mike A, Mitsuyama K, Sako T, Yamamoto M, Kado S, Takada T
Immunology 2009 Sep;128(1 Suppl):e170-80
Immunology 2009 Sep;128(1 Suppl):e170-80
Human mast cell activation with virus-associated stimuli leads to the selective chemotaxis of natural killer cells by a CXCL8-dependent mechanism.
Burke SM, Issekutz TB, Mohan K, Lee PW, Shmulevitz M, Marshall JS
Blood 2008 Jun 15;111(12):5467-76
Blood 2008 Jun 15;111(12):5467-76
IgE modulates neutrophil survival in asthma: role of mitochondrial pathway.
Saffar AS, Alphonse MP, Shan L, Hayglass KT, Simons FE, Gounni AS
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 15;178(4):2535-41
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 15;178(4):2535-41
IgE modulates neutrophil survival in asthma: role of mitochondrial pathway.
Saffar AS, Alphonse MP, Shan L, Hayglass KT, Simons FE, Gounni AS
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 15;178(4):2535-41
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 15;178(4):2535-41
Protein microarray platform for the multiplex analysis of biomarkers in human sera.
Urbanowska T, Mangialaio S, Zickler C, Cheevapruk S, Hasler P, Regenass S, Legay F
Journal of immunological methods 2006 Oct 20;316(1-2):1-7
Journal of immunological methods 2006 Oct 20;316(1-2):1-7
Probiotic Lactobacillus-induced improvement in murine chronic inflammatory bowel disease is associated with the down-regulation of pro-inflammatory cytokines in lamina propria mononuclear cells.
Matsumoto S, Hara T, Hori T, Mitsuyama K, Nagaoka M, Tomiyasu N, Suzuki A, Sata M
Clinical and experimental immunology 2005 Jun;140(3):417-26
Clinical and experimental immunology 2005 Jun;140(3):417-26
Tumor-infiltrating lymphocyte secretion of IL-6 antagonizes tumor-derived TGF-beta 1 and restores the lymphokine-activated killing activity.
Hsiao YW, Liao KW, Hung SW, Chu RM
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 1;172(3):1508-14
Journal of immunology (Baltimore, Md. : 1950) 2004 Feb 1;172(3):1508-14
Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis.
Schotte H, Schlüter B, Willeke P, Mickholz E, Schorat MA, Domschke W, Gaubitz M
Rheumatology (Oxford, England) 2004 Aug;43(8):960-4
Rheumatology (Oxford, England) 2004 Aug;43(8):960-4
Interleukin-6 fused to an anti-idiotype antibody in a vaccine increases the specific humoral immune response against CA125+ (MUC-16) ovarian cancer.
Reinartz S, Hombach A, Köhler S, Schlebusch H, Wallwiener D, Abken H, Wagner U
Cancer research 2003 Jun 15;63(12):3234-40
Cancer research 2003 Jun 15;63(12):3234-40
A nonsteroidal glucocorticoid receptor antagonist.
Miner JN, Tyree C, Hu J, Berger E, Marschke K, Nakane M, Coghlan MJ, Clemm D, Lane B, Rosen J
Molecular endocrinology (Baltimore, Md.) 2003 Jan;17(1):117-27
Molecular endocrinology (Baltimore, Md.) 2003 Jan;17(1):117-27
Serum cytokine levels, cigarette smoking and airway responsiveness among pregnant women.
Tsunoda M, Litonjua AA, Kuniak MP, Weiss ST, Satoh T, Guevarra L, Tollerud DJ
International archives of allergy and immunology 2003 Feb;130(2):158-64
International archives of allergy and immunology 2003 Feb;130(2):158-64
Ambient air particulates stimulate alveolar macrophages of smokers to promote differentiation of myeloid precursor cells.
Suwa T, Hogg JC, Vincent R, Mukae H, Fujii T, van Eeden SF
Experimental lung research 2002 Jan-Feb;28(1):1-18
Experimental lung research 2002 Jan-Feb;28(1):1-18
Ambient air particulates stimulate alveolar macrophages of smokers to promote differentiation of myeloid precursor cells.
Suwa T, Hogg JC, Vincent R, Mukae H, Fujii T, van Eeden SF
Experimental lung research 2002 Jan-Feb;28(1):1-18
Experimental lung research 2002 Jan-Feb;28(1):1-18
Release of vasoactive cytokines by antibody-enhanced dengue virus infection of a human mast cell/basophil line.
King CA, Marshall JS, Alshurafa H, Anderson R
Journal of virology 2000 Aug;74(15):7146-50
Journal of virology 2000 Aug;74(15):7146-50
Release of vasoactive cytokines by antibody-enhanced dengue virus infection of a human mast cell/basophil line.
King CA, Marshall JS, Alshurafa H, Anderson R
Journal of virology 2000 Aug;74(15):7146-50
Journal of virology 2000 Aug;74(15):7146-50
Regulation of interleukin-12 by complement receptor 3 signaling.
Marth T, Kelsall BL
The Journal of experimental medicine 1997 Jun 2;185(11):1987-95
The Journal of experimental medicine 1997 Jun 2;185(11):1987-95
Regulation of interleukin-12 by complement receptor 3 signaling.
Marth T, Kelsall BL
The Journal of experimental medicine 1997 Jun 2;185(11):1987-95
The Journal of experimental medicine 1997 Jun 2;185(11):1987-95
Sustained high levels of circulating chaperoned interleukin-6 after active specific cancer immunotherapy.
May LT, Patel K, García D, Ndubuisi MI, Ferrone S, Mittelman A, Mackiewicz A, Sehgal PB
Blood 1994 Sep 15;84(6):1887-95
Blood 1994 Sep 15;84(6):1887-95
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA analysis of human IL-6 was performed using a Human IL-6 Colorimetric ELISA kit (Product # EH2IL6) by loading 50 µL per well of a mouse anti-human IL-6 biotinylated antibody (Product # M621B) at a concentration of 0.035 µg/mL and 50 µL of Human IL-6 Recombinant Protein (Product # SIL6) at 400, 160, 64, 25.6, 10.24, and 0 pg/mL (both in quadruplicate) across a 4 µg/mL mouse anti-human IL-6 (Product # M620) pre-coated plate. The plate was incubated for 2 hours at room temperature, washed, and incubated with 100 µL per well of Streptavidin-HRP (Product # N504) in all test wells at 1:7000 for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB Substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2. NF-kappaB1, IL-6, MMP-13 and caspase-3 expression in the in vivo model of OA. (A) The relative protein expression levels of NF-kappaB1, IL-6, MMP-13 and caspase-3 were determined by western blot analysis in tissue samples from mice in the Sham, OA and OA + Res groups. Quantification of (B) NF-kappaB1, (C) IL-6, (D) MMP-13 and (E) caspase-3 protein expression. *P
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7. Effect of Res treatment on miR-9, NF-kappaB1, IL-6, MMP-13, caspase-3 expression in mouse chondrocytes. The relative (A) miR-9 and (B) NF-kappaB1 mRNA expression levels were determined by RT-qPCR in chondrocytes following treatment with 0, 15 or 30 mM Res for 48 h. (C) The relative NF-kappaB1, IL-6, MMP-13 and caspase-3 protein expression levels were determined by western blot analysis in chondrocytes following treatment with 0, 15 or 30 mM Res for 48 h. Quantification of (D) NF-kappaB1, (E) IL-6, (F) MMP-13 and (G) caspase-3 protein expression. n=3. *P
- Conjugate
- Biotin