Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF1609 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-6 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects canine IL-6 in ELISAs and Western blots. In sandwich ELISAs, less than 5% cross-reactivity with recombinant feline IL-6 and recombinant porcine IL-6 is observed, and less than 0.2% cross-reactivity with recombinant human IL-6, recombinant mouse IL-6, recombinant rat IL-6, recombinant cotton rat IL-6, and recombinant equine IL-6 is observed.
- Reactivity
- Canine
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of IL-6 in CLL-1390 Canine Cell Line by Flow Cytometry. CLL-1390 canine leukocytic round cell neoplasia cell line was stained with Goat Anti-Canine IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1609, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-6 and Neutralization by Canine IL-6 Antibody. Recombinant Canine IL-6 (Catalog # 1609-CL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-6 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1609). The ND50 is typically 0.4-2.0 µg/mL.