Antibody data
- Antibody Data
- Antigen structure
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- Validations
- ELISA [1]
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Validation data
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- Product number
- GTX15762 - Provider product page
- Provider
- GeneTex
- Product name
- IL6 antibody [5IL6]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Porcine
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sandwich ELISA analysis of human IL6 at 400, 160, 64, 25.6, 10.24, and 0 pg/ml (50 ul per well) using IL-6 antibody [7IL6] as capture antibody (4 ug/ml) and IL-6 antibody [5IL6] (Biotin) as detection antibody (0.035 ug/ml). The plate was washed and incubated with Streptavidin-HRP. Detection was performed using TMB substrate. The plate was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.