Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- IC2062G-025 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-6 Alexa Fluor® 488-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IL-6 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Antigen sequence
Q75MH2
- Isotype
- IgG
- Antibody clone number
- 903129
- Vial size
- 25 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-6 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) (A) untreated or (B) treated overnight with 250 ng/mL LPS were stained with Mouse Anti-Human IL-6 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC2062G) and Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A). Quadrant markers were set based on control antibody staining (Catalog # IC002A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.