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- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Immunocytochemistry [1]
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- Product number
- MAB686 - Provider product page
- Provider
- R&D Systems
- Product name
- Porcine IL-6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects porcine IL-6 in direct ELISAs and Western blots. Approximately 2% cross-reactivity with recombinant mouse (rm) IL-6, recombinant rat IL-6, and rmLIF is observed.
- Reactivity
- Porcine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P26893- Isotype
- IgG
- Antibody clone number
- 77830
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Characterization of a Broadly Reactive Anti-CD40 Agonistic Monoclonal Antibody for Potential Use as an Adjuvant.
Altered expression of cytokines IL-1α, IL-6, IL-8 and TNF-α in bovine follicular persistence.
Dendritic Cell Sensing of Hydrophobic Di- and Triacylated Lipopeptides Self-Assembled within Synthetic Virus-like Particles.
Mutations in classical swine fever virus NS4B affect virulence in swine.
Low vasa vasorum densities correlate with inflammation and subintimal thickening: potential role in location--determination of atherogenesis.
Sex difference in link between interleukin-6 and stress.
Martin C, Waghela SD, Lokhandwala S, Ambrus A, Bray J, Vuong C, Vinodkumar V, Dominowski PJ, Rai S, Mwangi D, Foss DL, Mwangi W
PloS one 2017;12(1):e0170504
PloS one 2017;12(1):e0170504
Altered expression of cytokines IL-1α, IL-6, IL-8 and TNF-α in bovine follicular persistence.
Stassi AF, Baravalle ME, Belotti EM, Rey F, Gareis NC, Díaz PU, Rodríguez FM, Leiva CJ, Ortega HH, Salvetti NR
Theriogenology 2017 Jul 15;97:104-112
Theriogenology 2017 Jul 15;97:104-112
Dendritic Cell Sensing of Hydrophobic Di- and Triacylated Lipopeptides Self-Assembled within Synthetic Virus-like Particles.
Sharma R, Ghasparian A, Robinson JA, McCullough KC
Journal of immunology (Baltimore, Md. : 1950) 2017 Jul 15;199(2):734-749
Journal of immunology (Baltimore, Md. : 1950) 2017 Jul 15;199(2):734-749
Mutations in classical swine fever virus NS4B affect virulence in swine.
Fernandez-Sainz I, Gladue DP, Holinka LG, O'Donnell V, Gudmundsdottir I, Prarat MV, Patch JR, Golde WT, Lu Z, Zhu J, Carrillo C, Risatti GR, Borca MV
Journal of virology 2010 Feb;84(3):1536-49
Journal of virology 2010 Feb;84(3):1536-49
Low vasa vasorum densities correlate with inflammation and subintimal thickening: potential role in location--determination of atherogenesis.
Gössl M, Versari D, Lerman LO, Chade AR, Beighley PE, Erbel R, Ritman EL
Atherosclerosis 2009 Oct;206(2):362-8
Atherosclerosis 2009 Oct;206(2):362-8
Sex difference in link between interleukin-6 and stress.
Jankord R, Turk JR, Schadt JC, Casati J, Ganjam VK, Price EM, Keisler DH, Laughlin MH
Endocrinology 2007 Aug;148(8):3758-64
Endocrinology 2007 Aug;148(8):3758-64
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-6 in Porcine PBMCs. IL-6 was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Porcine IL-6 Monoclonal Antibody (Catalog # MAB686) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
