Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- MAB16091 - Provider product page
- Provider
- R&D Systems
- Product name
- Canine IL-6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects canine IL-6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10%-50% cross-reactivity with recombinant eqine IL-6, recombinant human (rh) IL-6, recombinant mouse (rm) IL-6, recombinant porcine IL-6, and recombinant rat (rr) IL-6 is observed and no cross-reactivity with rmCardiotrophin-1, rhCLC, rrCNTF, recombinant cotton rat IL-6, rmIL-11, rmLIF, or rmOncostatin M is observed.
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P41323
- Isotype
- IgG
- Antibody clone number
- 247002
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IL-6 in Canine PBMCs. IL-6 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Canine IL-6 Monoclonal Antibody (Catalog # MAB16091) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.