Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX84294 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84294, RRID:AB_10729634
- Product name
- IL6 antibody [3H6]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Storage
- For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IL-6 antibody (3H6) at 1:2000 + recombinant human IL-6
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-6.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-6.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-IL-6 mouse monoclonal antibody (GTX84294) immunofluorescent staining of HELA cells transiently transfected with IL-6