GTX84294

antibody from GeneTex

Targeting: IL6 BSF2, HGF, HSF, IFNB2, IL-6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX84294

Provider

GeneTex

Proper citation

GeneTex Cat#GTX84294, RRID:AB_10729634

Product name

IL6 antibody [3H6]

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Storage

For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IL-6 antibody (3H6) at 1:2000 + recombinant human IL-6

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-6.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-6.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Anti-IL-6 mouse monoclonal antibody (GTX84294) immunofluorescent staining of HELA cells transiently transfected with IL-6