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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [25]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-406-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-6 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse IL-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant rat IL-6 and recombinant cotton rat IL-6 is observed and less than 15% cross-reactivity with recombinant human IL-6 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P08505- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references RIPK1 inhibition attenuates experimental autoimmune arthritis via suppression of osteoclastogenesis.
3-(1H-Benzo[d]imidazol-6-yl)-5-(4-fluorophenyl)-1,2,4-oxadiazole (DDO7232), a Novel Potent Nrf2/ARE Inducer, Ameliorates DSS-Induced Murine Colitis and Protects NCM460 Cells against Oxidative Stress via ERK1/2 Phosphorylation.
Evidence for acute contraction-induced myokine secretion by C2C12 myotubes.
BATF regulates collagen-induced arthritis by regulating T helper cell differentiation.
Inhibiting the NLRP3 inflammasome with MCC950 promotes non-phlogistic clearance of amyloid-β and cognitive function in APP/PS1 mice.
CD8+ T cells produce a dialyzable antigen-specific activator of dendritic cells.
Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism.
Respiratory tract epithelial cells express retinaldehyde dehydrogenase ALDH1A and enhance IgA production by stimulated B cells in the presence of vitamin A.
Dysregulation of suppressor of cytokine signaling 3 in keratinocytes causes skin inflammation mediated by interleukin-20 receptor-related cytokines.
IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells.
Tumor development in murine ulcerative colitis depends on MyD88 signaling of colonic F4/80+CD11b(high)Gr1(low) macrophages.
Foxa1 and Foxa2 regulate bile duct development in mice.
Uropathogenic Escherichia coli-induced inflammation alters mouse urinary bladder contraction via an interleukin-6-activated inducible nitric oxide synthase-related pathway.
The critical role of invading peripheral macrophage-derived interleukin-6 in vincristine-induced mechanical allodynia in mice.
Neuritogenic effects of T cell-derived IL-3 on mouse splenic sympathetic neurons in vivo.
Neuritogenic effects of T cell-derived IL-3 on mouse splenic sympathetic neurons in vivo.
Particulate matter exposure induces persistent lung inflammation and endothelial dysfunction.
Mechanisms of interleukin-6 synthesis and release induced by interleukin-1 and cell depolarisation in neurones.
Interleukin-6 as a mechanism for the adverse effects of social stress on acute Theiler's virus infection.
Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells.
High pressure promotes monocyte adhesion to the vascular wall.
The sex steroid precursor DHEA accelerates cutaneous wound healing via the estrogen receptors.
The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
Jhun J, Lee SH, Kim SY, Ryu J, Kwon JY, Na HS, Jung K, Moon SJ, Cho ML, Min JK
Journal of translational medicine 2019 Mar 15;17(1):84
Journal of translational medicine 2019 Mar 15;17(1):84
3-(1H-Benzo[d]imidazol-6-yl)-5-(4-fluorophenyl)-1,2,4-oxadiazole (DDO7232), a Novel Potent Nrf2/ARE Inducer, Ameliorates DSS-Induced Murine Colitis and Protects NCM460 Cells against Oxidative Stress via ERK1/2 Phosphorylation.
Xu LL, Liu T, Wang L, Li L, Wu YF, Li CC, Di B, You QD, Jiang ZY
Oxidative medicine and cellular longevity 2018;2018:3271617
Oxidative medicine and cellular longevity 2018;2018:3271617
Evidence for acute contraction-induced myokine secretion by C2C12 myotubes.
Furuichi Y, Manabe Y, Takagi M, Aoki M, Fujii NL
PloS one 2018;13(10):e0206146
PloS one 2018;13(10):e0206146
BATF regulates collagen-induced arthritis by regulating T helper cell differentiation.
Park SH, Rhee J, Kim SK, Kang JA, Kwak JS, Son YO, Choi WS, Park SG, Chun JS
Arthritis research & therapy 2018 Aug 2;20(1):161
Arthritis research & therapy 2018 Aug 2;20(1):161
Inhibiting the NLRP3 inflammasome with MCC950 promotes non-phlogistic clearance of amyloid-β and cognitive function in APP/PS1 mice.
Dempsey C, Rubio Araiz A, Bryson KJ, Finucane O, Larkin C, Mills EL, Robertson AAB, Cooper MA, O'Neill LAJ, Lynch MA
Brain, behavior, and immunity 2017 Mar;61:306-316
Brain, behavior, and immunity 2017 Mar;61:306-316
CD8+ T cells produce a dialyzable antigen-specific activator of dendritic cells.
Myles IA, Zhao M, Nardone G, Olano LR, Reckhow JD, Saleem D, Break TJ, Lionakis MS, Myers TG, Gardina PJ, Kirkpatrick CH, Holland SM, Datta SK
Journal of leukocyte biology 2017 Jan;101(1):307-320
Journal of leukocyte biology 2017 Jan;101(1):307-320
Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism.
Zhao C, Zhang W, Zhao Y, Yang Y, Luo H, Ji G, Dong E, Deng H, Lin S, Wei Y, Yang H
Scientific reports 2016 Jan 14;6:19404
Scientific reports 2016 Jan 14;6:19404
Respiratory tract epithelial cells express retinaldehyde dehydrogenase ALDH1A and enhance IgA production by stimulated B cells in the presence of vitamin A.
Rudraraju R, Jones BG, Surman SL, Sealy RE, Thomas PG, Hurwitz JL
PloS one 2014;9(1):e86554
PloS one 2014;9(1):e86554
Dysregulation of suppressor of cytokine signaling 3 in keratinocytes causes skin inflammation mediated by interleukin-20 receptor-related cytokines.
Uto-Konomi A, Miyauchi K, Ozaki N, Motomura Y, Suzuki Y, Yoshimura A, Suzuki S, Cua D, Kubo M
PloS one 2012;7(7):e40343
PloS one 2012;7(7):e40343
IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells.
Fisher DT, Chen Q, Skitzki JJ, Muhitch JB, Zhou L, Appenheimer MM, Vardam TD, Weis EL, Passanese J, Wang WC, Gollnick SO, Dewhirst MW, Rose-John S, Repasky EA, Baumann H, Evans SS
The Journal of clinical investigation 2011 Oct;121(10):3846-59
The Journal of clinical investigation 2011 Oct;121(10):3846-59
Tumor development in murine ulcerative colitis depends on MyD88 signaling of colonic F4/80+CD11b(high)Gr1(low) macrophages.
Schiechl G, Bauer B, Fuss I, Lang SA, Moser C, Ruemmele P, Rose-John S, Neurath MF, Geissler EK, Schlitt HJ, Strober W, Fichtner-Feigl S
The Journal of clinical investigation 2011 May;121(5):1692-708
The Journal of clinical investigation 2011 May;121(5):1692-708
Foxa1 and Foxa2 regulate bile duct development in mice.
Li Z, White P, Tuteja G, Rubins N, Sackett S, Kaestner KH
The Journal of clinical investigation 2009 Jun;119(6):1537-45
The Journal of clinical investigation 2009 Jun;119(6):1537-45
Uropathogenic Escherichia coli-induced inflammation alters mouse urinary bladder contraction via an interleukin-6-activated inducible nitric oxide synthase-related pathway.
Weng TI, Wu HY, Lin PY, Liu SH
Infection and immunity 2009 Aug;77(8):3312-9
Infection and immunity 2009 Aug;77(8):3312-9
The critical role of invading peripheral macrophage-derived interleukin-6 in vincristine-induced mechanical allodynia in mice.
Kiguchi N, Maeda T, Kobayashi Y, Kondo T, Ozaki M, Kishioka S
European journal of pharmacology 2008 Sep 11;592(1-3):87-92
European journal of pharmacology 2008 Sep 11;592(1-3):87-92
Neuritogenic effects of T cell-derived IL-3 on mouse splenic sympathetic neurons in vivo.
Kannan-Hayashi Y, Okamura K, Hattori S, Kuwamura M, Higuchi E, Terayama H, Moriyama M, Mukamoto M, Okada M, Ohsugi Y, Nakamura Y
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 15;180(6):4227-34
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 15;180(6):4227-34
Neuritogenic effects of T cell-derived IL-3 on mouse splenic sympathetic neurons in vivo.
Kannan-Hayashi Y, Okamura K, Hattori S, Kuwamura M, Higuchi E, Terayama H, Moriyama M, Mukamoto M, Okada M, Ohsugi Y, Nakamura Y
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 15;180(6):4227-34
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 15;180(6):4227-34
Particulate matter exposure induces persistent lung inflammation and endothelial dysfunction.
Tamagawa E, Bai N, Morimoto K, Gray C, Mui T, Yatera K, Zhang X, Xing L, Li Y, Laher I, Sin DD, Man SF, van Eeden SF
American journal of physiology. Lung cellular and molecular physiology 2008 Jul;295(1):L79-85
American journal of physiology. Lung cellular and molecular physiology 2008 Jul;295(1):L79-85
Mechanisms of interleukin-6 synthesis and release induced by interleukin-1 and cell depolarisation in neurones.
Tsakiri N, Kimber I, Rothwell NJ, Pinteaux E
Molecular and cellular neurosciences 2008 Jan;37(1):110-8
Molecular and cellular neurosciences 2008 Jan;37(1):110-8
Interleukin-6 as a mechanism for the adverse effects of social stress on acute Theiler's virus infection.
Meagher MW, Johnson RR, Young EE, Vichaya EG, Lunt S, Hardin EA, Connor MA, Welsh CJ
Brain, behavior, and immunity 2007 Nov;21(8):1083-95
Brain, behavior, and immunity 2007 Nov;21(8):1083-95
Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells.
Kurooka M, Kaneda Y
Cancer research 2007 Jan 1;67(1):227-36
Cancer research 2007 Jan 1;67(1):227-36
High pressure promotes monocyte adhesion to the vascular wall.
Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S
Circulation research 2007 Apr 27;100(8):1226-33
Circulation research 2007 Apr 27;100(8):1226-33
The sex steroid precursor DHEA accelerates cutaneous wound healing via the estrogen receptors.
Mills SJ, Ashworth JJ, Gilliver SC, Hardman MJ, Ashcroft GS
The Journal of investigative dermatology 2005 Nov;125(5):1053-62
The Journal of investigative dermatology 2005 Nov;125(5):1053-62
The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M
Archives of dermatological research 2001 Nov;293(10):508-14
Archives of dermatological research 2001 Nov;293(10):508-14
The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M
Archives of dermatological research 2001 Nov;293(10):508-14
Archives of dermatological research 2001 Nov;293(10):508-14
A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
Shih CC, Hu MC, Hu J, Weng Y, Yazaki PJ, Medeiros J, Forman SJ
Blood 2000 Mar 15;95(6):1957-66
Blood 2000 Mar 15;95(6):1957-66
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse IL-6 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-406-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-6 in Mouse Thymus. IL-6 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-406-NA) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-6 and Neutralization by Mouse IL-6 Antibody. Recombinant Mouse IL-6 (Catalog # 406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-406-NA). The ND50 is typically 0.01-0.03 µg/mL.
