H00003569-M10

antibody from Abnova Corporation

Targeting: IL6 BSF2, HGF, HSF, IFNB2, IL-6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003569-M10

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003569-M10, RRID:AB_626469

Product name

IL6 monoclonal antibody (M10), clone 4B12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant IL6.

Antigen sequence

SPVPPGEDSKDVAAPHRQPLTSSERIDKQIRYILD
GISALRKETCNKSNMCESSKEALAENNLNLPKMAE
KDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNR
FESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTP
DPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFL
QSSLRALRQM


Isotype

IgG

Antibody clone number

4B12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of IL6 expression in transfected 293T cell line by IL6 monoclonal antibody (M10), clone 4B12.Lane 1: IL6 transfected lysate(23.7 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to IL6 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol