TA500071

antibody from OriGene

Targeting: IL6 BSF2, HGF, HSF, IFNB2, IL-6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500071

Provider

OriGene

Proper citation

OriGene Cat#TA500071, RRID:AB_2280507

Product name

IL6 mouse monoclonal antibody, clone OTI3H6 (formerly 3H6)

Antibody type

Monoclonal

Description

IL6 mouse monoclonal antibody, clone OTI3H6 (formerly 3H6)

Host

Mouse

Conjugate

Unconjugated

Epitope

IL6

Isotype

IgG

Antibody clone number

OTI3H6

Vial size

100 µl

Concentration

0.24 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

IL-6 antibody (3H6) at 1:2000 + recombinant human IL-6 (Cat# RC202078)

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-6 (RC202078, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-6.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-IL-6 mouse monoclonal antibody (TA500071) immunofluorescent staining of HELA cells transiently transfected by pCMV6-ENTRY IL-6(RC202078).

Validation comment

IF