209-301-310

antibody from Rockland Immunochemicals, Inc.

Targeting: IL6 BSF2, HGF, HSF, IFNB2, IL-6

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

209-301-310

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#209-301-310, RRID:AB_11183337

Product name

Anti-Human IL-6 (MOUSE) Monoclonal Antibody - 209-301-310

Antibody type

Monoclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western Blot showing detection of Human IL-6. 100 ng of Human IL-6 was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C, Anti-Human IL-6 (MOUSE) Antibody (p/n 209-301-310) was used at 1:1000 in 1% BSA-TTBS over night at 4°C. Peroxidase conjugated Rabbit Anti-mouse secondary antibody was used in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) at 1:40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc® 4000 MP. Band indicates correct 17 kDa molecular weight position expected for Human IL-6.

Validation comment

Western Blot