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Learn209-301-310S
antibody from Rockland Immunochemicals, Inc.
Targeting: IL6
BSF2, HGF, HSF, IFNB2, IL-6
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 209-301-310S - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#209-301-310S, RRID:AB_11181443
- Product name
- Anti-Human IL-6 (MOUSE) Monoclonal Antibody - 209-301-310S
- Antibody type
- Monoclonal
- Vial size
- 25 µl
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot showing detection of Human IL-6. 100 ng of Human IL-6 was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C, Anti-Human IL-6 (MOUSE) Antibody (p/n 209-301-310) was used at 1:1000 in 1% BSA-TTBS over night at 4°C. Peroxidase conjugated Rabbit Anti-mouse secondary antibody was used in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) at 1:40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc® 4000 MP. Band indicates correct 17 kDa molecular weight position expected for Human IL-6.
- Validation comment
- Western Blot
