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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- 501113 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#501113, RRID:AB_2561425
- Product name
- Pacific Blue™ anti-human IL-6 [MQ2-13A5]
- Antibody type
- Monoclonal
- Antigen
- COS-7- expressed, recombinant human IL-6
- Description
- IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Blue dye
- Isotype
- IgG
- Vial size
- 25 tests
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Submitted references Induction of IL-6 in transcriptional networks in corneal epithelial cells after herpes simplex virus type 1 infection.
Terasaka Y, Miyazaki D, Yakura K, Haruki T, Inoue Y
Investigative ophthalmology & visual science 2010 May;51(5):2441-9
Investigative ophthalmology & visual science 2010 May;51(5):2441-9
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- LPS-stimulated human peripheral blood monocytes (for 6 hours in the presence of monensin) were stained with CD14 APC, fixed, permeabilized, and then stained with IL-6 (clone MQ2-13A5) Pacific Blue™ (top) or rat IgG1, κ Pacific Blue™ isotype control (bottom).
- Conjugate
- Blue dye
