R1134

antibody from Acris Antibodies GmbH

Targeting: IL6 BSF2, HGF, HSF, IFNB2, IL-6

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Functional assay (Recommended by provider)

Antibody Data

Product number

R1134

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1134, RRID:AB_1004120

Product name

anti Interleukin-6 (IL6)

Antibody type

Polyclonal

Antigen

Recombinant Human IL-6 produced in E.coli.

Reactivity

Human

Host

Rabbit

Vial size

1 ml

Concentration

80 mg/ml (by refractometry)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using R1134 IL6 antibody. Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band ~21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG (R1454HRP) secondary antibody diluted1/40,000 in blocking buffer (p/n MB-070) for 30 min at RTfollowed by reaction with FemtoMax™ chemiluminescentsubstrate. Image was captured using VersaDoc™ MP 4000imaging system (Bio-Rad).