600-401-853

antibody from Rockland Immunochemicals, Inc.

Targeting: GGA1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-853

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-853, RRID:AB_2110594

Product name

Anti-GGA1 (RABBIT) Antibody - 600-401-853

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's Affinity Purified anti-GGA1 antibody shows detection of bands at ~100 kDa corresponding to YFP-GGA1 fusion present in a lysate of HEK293 cells over- expressing the recombinant protein (arrowhead).  Approximately 35 µg of lysate was separated on a 4-20% gel by SDS-PAGE and transferred onto nitrocellulose.   After blocking the membrane was probed with the primary antibody diluted to 1:1,350. Reaction occurred overnight at 4° followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MXHu (605-432-013) for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.

Validation comment

Western Blot