Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- M29-652R - Provider product page
- Provider
- SignalChem
- Product name
- Anti-phospho-ERK1/2 (Thr202/Tyr204)
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Purified by affinity chromatography
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Simian, Xenopus, Zebrafish
- Host
- Rabbit
- Epitope
- Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 conjugated to KLH
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- SignalChem (provider)
- Main image
- Experimental details
- Western blot of human T47D cell lysates showing specific immunolabeling of ~42-44kDa ERK1/2 protein phosphorylated at Thr202/Tyr204 (Control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-phospho-ERK1/2 (Thr202/Tyr204) antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
- Sample type
- Western Blot
Supportive validation
- Submitted by
- SignalChem (provider)
- Main image
- Experimental details
- Immunostaining of granule cells in the dentate gyrus of saline treated mouse showing ERK1/2 when phosphorylated at Thr202/Tyr204 (red) and nuclei (blue). Photo courtesy of Robert Wine.
- Sample type
- Immunostaining
