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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB10800 - Provider product page
- Provider
- Abnova Corporation
- Product name
- MAPK3 monoclonal antibody, clone 1E5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant MAPK3.
- Isotype
- IgG
- Antibody clone number
- 1E5
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using MAPK3 monoclonal antibody, clone 1E5 (Cat # MAB10800) against HeLa (1), Jurkat (2), RAW264.7 (3), HEK293 (4), K-562 (5), NIH/3T3 (6), Cos7 (7) and PC-12 (8) cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using MAPK3 monoclonal antibody, clone 1E5 (Cat # MAB10800) against human MAPK3 (aa: 9-143) recombinant protein. (Expected MW is 40.8 kDa)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA measurement of MAPK3 monoclonal antibody, clone 1E5 (Cat # MAB10800).
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NIH/3T3 cells using MAPK3 monoclonal antibody, clone 1E5 (Cat # MAB10800) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MAPK3 monoclonal antibody, clone 1E5 (Cat # MAB10800) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer (A) and human bladder cancer (B) tissues using MAPK3 monoclonal antibody, clone 1E5 (Cat # MAB10800) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using MAPK3 monoclonal antibody, clone 1E5 (Cat # MAB10800) (blue) and negative control (red).
- Validation comment
- Flow Cytometry
