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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [12]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- bs-3016R - Provider product page
- Provider
- Bioss
- Proper citation
- Bioss Cat#bs-3016R, RRID:AB_10855752
- Product name
- ERK1/2(Thr202 + Tyr204) Antibody
- Antibody type
- Polyclonal
- Description
- KLH conjugated synthetic phosphopeptide derived from human p44/42 MAPK around the phosphorylation site of (Thr202/Tyr204)
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1ug/ul
- Storage
- Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Submitted references Effect of lncRNA ANRIL knockdown on proliferation and cisplatin chemoresistance of osteosarcoma cells in vitro.
Dietary l-tryptophan alleviated LPS-induced intestinal barrier injury by regulating tight junctions in a Caco-2 cell monolayer model.
Lysine Enhances the Stimulation of Fatty Acids on Milk Fat Synthesis via the GPRC6A-PI3K-FABP5 Signaling in Bovine Mammary Epithelial Cells.
LncIRS1 controls muscle atrophy via sponging miR-15 family to activate IGF1-PI3K/AKT pathway.
LncRNA CASC2 inhibits proliferation and migration of adenocarcinoma cells via miR-4735-3p and mTOR.
LncRNA as ceRNAs may be involved in lactation process.
Positive prognostic value of HER2-HER3 co-expression and p-mTOR in gastric cancer patients.
PGC-1α modulates necrosis, inflammatory response, and fibrotic tissue formation in injured skeletal muscle.
Transplantation of Autologous Bone Marrow Mononuclear Cells Regulates Inflammation in a Rabbit Model of Carotid Artery Atherosclerosis.
P62 regulates resveratrol-mediated Fas/Cav-1 complex formation and transition from autophagy to apoptosis.
Expression of vascular cell adhesion molecule-1 in the aortic tissues of atherosclerotic patients and the associated clinical implications.
Upregulation of progranulin by Helicobacter pylori in human gastric epithelial cells via p38MAPK and MEK1/2 signaling pathway: role in epithelial cell proliferation and migration.
Li G, Zhu Y
Pathology, research and practice 2019 May;215(5):931-938
Pathology, research and practice 2019 May;215(5):931-938
Dietary l-tryptophan alleviated LPS-induced intestinal barrier injury by regulating tight junctions in a Caco-2 cell monolayer model.
Chen M, Liu Y, Xiong S, Wu M, Li B, Ruan Z, Hu X
Food & function 2019 May 22;10(5):2390-2398
Food & function 2019 May 22;10(5):2390-2398
Lysine Enhances the Stimulation of Fatty Acids on Milk Fat Synthesis via the GPRC6A-PI3K-FABP5 Signaling in Bovine Mammary Epithelial Cells.
Li X, Li P, Wang L, Zhang M, Gao X
Journal of agricultural and food chemistry 2019 Jun 26;67(25):7005-7015
Journal of agricultural and food chemistry 2019 Jun 26;67(25):7005-7015
LncIRS1 controls muscle atrophy via sponging miR-15 family to activate IGF1-PI3K/AKT pathway.
Li Z, Cai B, Abdalla BA, Zhu X, Zheng M, Han P, Nie Q, Zhang X
Journal of cachexia, sarcopenia and muscle 2019 Apr;10(2):391-410
Journal of cachexia, sarcopenia and muscle 2019 Apr;10(2):391-410
LncRNA CASC2 inhibits proliferation and migration of adenocarcinoma cells via miR-4735-3p and mTOR.
Luo M, Kong D, Pei D, Jin X, Liu D
Journal of cellular biochemistry 2018 Nov 18;
Journal of cellular biochemistry 2018 Nov 18;
LncRNA as ceRNAs may be involved in lactation process.
Yu S, Zhao Y, Lai F, Chu M, Hao Y, Feng Y, Zhang H, Liu J, Cheng M, Li L, Shen W, Min L
Oncotarget 2017 Nov 17;8(58):98014-98028
Oncotarget 2017 Nov 17;8(58):98014-98028
Positive prognostic value of HER2-HER3 co-expression and p-mTOR in gastric cancer patients.
Cao GD, Chen K, Chen B, Xiong MM
BMC cancer 2017 Dec 12;17(1):841
BMC cancer 2017 Dec 12;17(1):841
PGC-1α modulates necrosis, inflammatory response, and fibrotic tissue formation in injured skeletal muscle.
Dinulovic I, Furrer R, Di Fulvio S, Ferry A, Beer M, Handschin C
Skeletal muscle 2016;6:38
Skeletal muscle 2016;6:38
Transplantation of Autologous Bone Marrow Mononuclear Cells Regulates Inflammation in a Rabbit Model of Carotid Artery Atherosclerosis.
Cui K, Wang M, Yu L, Ren X, Cui H, Yu XF, Hou S, Fu C, Wang J
Journal of vascular research 2016;53(3-4):196-205
Journal of vascular research 2016;53(3-4):196-205
P62 regulates resveratrol-mediated Fas/Cav-1 complex formation and transition from autophagy to apoptosis.
Zhang J, Ma K, Qi T, Wei X, Zhang Q, Li G, Chiu JF
Oncotarget 2015 Jan 20;6(2):789-801
Oncotarget 2015 Jan 20;6(2):789-801
Expression of vascular cell adhesion molecule-1 in the aortic tissues of atherosclerotic patients and the associated clinical implications.
Mu W, Chen M, Gong Z, Zheng F, Xing Q
Experimental and therapeutic medicine 2015 Aug;10(2):423-428
Experimental and therapeutic medicine 2015 Aug;10(2):423-428
Upregulation of progranulin by Helicobacter pylori in human gastric epithelial cells via p38MAPK and MEK1/2 signaling pathway: role in epithelial cell proliferation and migration.
Wang H, Sun Y, Liu S, Yu H, Li W, Zeng J, Chen C, Jia J
FEMS immunology and medical microbiology 2011 Oct;63(1):82-92
FEMS immunology and medical microbiology 2011 Oct;63(1):82-92
No comments: Submit comment
Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Lane 1: human colon carcinoma lysates probed with Anti phospho-ERK1/2(Thr202 + Tyr204) Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37°C. Predicted band 44kD. Observed band size: 44kD
- Sample type
- Human
- Other comments
- Colon
Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded human liver labeled with Anti-phospho-ERK1/2(Thr202 + Tyr204) Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
- Sample type
- Human
- Other comments
- Liver
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-ERK1/2(Thr202 + Tyr204)Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37°C and DAPI staining
- Sample type
- Human
- Other comments
- Lung
