ARG51732

antibody from Arigo

Targeting: MAPK3 ERK1, p44erk1, p44mapk, PRKM3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ARG51732

Provider

Arigo

Product name

anti-p44/42 MAP Kinase phospho (Thr202) antibody

Antibody type

Polyclonal

Antigen

Peptide sequence around phosphorylation site of threonine 202 (F-L-T(p)-E-Y) derived from Human p44/42 MAP Kinase.

Description

Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µl

Concentration

1 mg/ml

Storage

Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western Blot: extracts from 293 cells untreated or treated with PMA for the indicated times, stained with anti-p44/42 MAP Kinase (phospho Thr202) antibody ARG51732.

Immunocytochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunofluorescence: methanol-fixed HeLa cells showing cytoplasmic, nuclear staining stained with anti-p44/42 MAP Kinase (phospho Thr202) antibody ARG51732.

Immunohistochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-p44/42 MAP Kinase (phospho Thr202) antibody ARG51732 (left) or the same antibody preincubated with blocking peptide (right).