686902

antibody from BioLegend

Targeting: MAPK3 ERK1, p44erk1, p44mapk, PRKM3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

686902

Provider

BioLegend

Proper citation

BioLegend Cat#686902, RRID:AB_2629535

Product name

Purified anti-ERK1/2

Antibody type

Monoclonal

Antigen

Partial human ERK1 recombinant protein (180-379 a.a.) expressed in E. coli.

Reactivity

Human, Mouse

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W15133B

Vial size

100 µg

Concentration

0.5 μ/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysate from human HeLa cells (lane 1), Jurkat cells (lane 2), and mouse NIH-3T3 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-ERK1/2 antibody (clone W15133B). Proteins were visualized using an HRP goat anti-rat IgG antibody and chemiluminescence detection.

Conjugate

Unconjugated