AM06672SU-N

antibody from OriGene

Targeting: MAPK3 ERK1, p44erk1, p44mapk, PRKM3

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM06672SU-N

Provider

OriGene

Product name

ERK1 (MAPK3) mouse monoclonal antibody, clone 1E5, Ascites

Antibody type

Monoclonal

Description

ERK1 (MAPK3) mouse monoclonal antibody, clone 1E5, Ascites

Host

Mouse

Conjugate

Unconjugated

Epitope

MAPK3

Isotype

IgG

Antibody clone number

1E5

Vial size

100 µl

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis using MAPK3 mAb against human MAPK3 (AA: 9-143) recombinant protein. (Expected MW is 40.8 kDa)

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis using MAPK3 mouse mAb against Hela (1), Jurkat (2), RAW264.7 (3), HEK293 (4), K562 (5), NIH/3T3 (6), Cos7 (7) and PC-12 (8) cell lysate.

Validation comment

WB

ELISA

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)

Validation comment

ELISA

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescence analysis of NIH/3T3 cells using MAPK3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using MAPK3 mouse mAb with DAB staining.

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using MAPK3 mouse mAb with DAB staining.

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric analysis of Hela cells using MAPK3 mouse mAb (blue) and negative control (red).

Validation comment

FC