Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB8305 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Rab13 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Rab13 in direct ELISA and Western Blot.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references RABIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis.
Gulbranson DR, Davis EM, Demmitt BA, Ouyang Y, Ye Y, Yu H, Shen J
Proceedings of the National Academy of Sciences of the United States of America 2017 Sep 26;114(39):E8224-E8233
Proceedings of the National Academy of Sciences of the United States of America 2017 Sep 26;114(39):E8224-E8233
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Detection of Human Rab13 by Western Blot. Western blot shows lysates of human lung tissue and human aorta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Rab13 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.