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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [2]
- Other assay [2]
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- Product number
- MA1-23294 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRIP230 Monoclonal Antibody (10G5)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Recommended positive controls: MCF7, HBL100, T24.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 10G5
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A mutation in VPS15 (PIK3R4) causes a ciliopathy and affects IFT20 release from the cis-Golgi.
Stoetzel C, Bär S, De Craene JO, Scheidecker S, Etard C, Chicher J, Reck JR, Perrault I, Geoffroy V, Chennen K, Strähle U, Hammann P, Friant S, Dollfus H
Nature communications 2016 Nov 24;7:13586
Nature communications 2016 Nov 24;7:13586
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TRIP230 in methanol-fixed HeLa cells using a TRIP230 monoclonal antibody (Product # MA1-23294) at a 1:100 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using TRIP230 (Product # MA1-23294) antibody at 1:100 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 GM130 interacts with VPS15 but not VPS34. ( a ) Control and patient fibroblasts were grown in complete medium (+FCS) or deprived of serum (-FCS) for 24 h. Immunoprecipitations with VPS15 or VPS34 antibodies were done on cell lysates and GM130 was detected by western blot. GAPDH was used as a loading control. ( b ) Control (ctrl) and patient (II.5) fibroblasts were grown and deprived of serum for 24 h. Immunofluorescence against VPS15 (red) and GM130 (green) and DAPI staining (blue) was performed and the cells were observed with a confocal microscope. ( c ) Control (ctrl) and patient (II.5) fibroblasts were grown in complete medium (+FCS) or deprived of serum (-FCS) for 24 h. Immunofluorescence against VPS15 (green) and GMAP210 (red) and DAPI staining (blue) was performed and the cells were observed with a confocal microscope.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 IFT20 is retained in the Golgi in patient fibroblasts. ( a ) Control (ctrl) and patient (II.5) fibroblasts were grown in complete medium (+FCS) or deprived of serum for 24 h (-FCS) and fixed. Immunofluorescence against IFT20 (red) and GM130 (green) and DAPI staining (blue) was performed and the cells were observed on a confocal microscope, only the merge (IFT20, GM130 and DAPI) is shown. Amount of red (IFT20) fluorescence in the cytoplasm was determined using ImageJ to measure the total amount of IFT20 fluorescence and substract fluorescence at the Golgi (green, GM130). The measures were done on 10 non-serum-deprived cells and 26 and 28 cells for ctrl and patient cells in serum-deprived conditions, respectively and error bars represent s.d. Mean fluorescence was calculated and statistical significance determined using a Student's t -test, ** P
