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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- ABIN1882065 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae) (ATG4B) (C-Term), (AA 358-390) antibody
- Antibody type
- Polyclonal
- Antigen
- This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-390 amino acids from the C-terminal region of human ATG4B.
- Description
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- C-Term,AA 358-390
- Antibody clone number
- RB7560
- Vial size
- 400 μL
- Concentration
- 2 mg/mL
Submitted references A high-throughput FRET-based assay for determination of Atg4 activity.
Kinetics comparisons of mammalian Atg4 homologues indicate selective preferences toward diverse Atg8 substrates.
Properties of human malignant glioma cells in vitro.
Determinants for the establishment of permanent tissue culture lines from human gliomas.
Li M, Chen X, Ye QZ, Vogt A, Yin XM
Autophagy 2012 Mar;8(3):401-12
Autophagy 2012 Mar;8(3):401-12
Kinetics comparisons of mammalian Atg4 homologues indicate selective preferences toward diverse Atg8 substrates.
Li M, Hou Y, Wang J, Chen X, Shao ZM, Yin XM
The Journal of biological chemistry 2011 Mar 4;286(9):7327-38
The Journal of biological chemistry 2011 Mar 4;286(9):7327-38
Properties of human malignant glioma cells in vitro.
Pontén J, Westermark B
Medical biology 1978 Aug;56(4):184-93
Medical biology 1978 Aug;56(4):184-93
Determinants for the establishment of permanent tissue culture lines from human gliomas.
Westermark B, Pontén J, Hugosson R
Acta pathologica et microbiologica Scandinavica. Section A, Pathology 1973 Nov;81(6):791-805
Acta pathologica et microbiologica Scandinavica. Section A, Pathology 1973 Nov;81(6):791-805
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of anti-hAPG4B-373 Pab (ABIN1882065) in 293 cell line lysates transiently transfected with the ATG4B gene (2 μg/lane). hAPG4B-373(arrow) was detected using the purified Pab.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of anti-hAPG4B-373 Pab (ABIN1882065) in 293 cell line lysates transiently transfected with the ATG4B gene (2 μg/lane). hAPG4B-373(arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of anti-hAPG4B-373 Pab (ABIN1882065) in 293 cell line lysates transiently transfected with the ATG4B gene (2 μg/lane). hAPG4B-373(arrow) was detected using the purified Pab.
