MA5-34792

antibody from Invitrogen Antibodies

Targeting: BUB3 BUB3L

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34792

Provider

Invitrogen Antibodies

Product name

Bub3 Recombinant Rabbit Monoclonal Antibody (JG38-81)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Control: A549, HL-60, A431, rat colon tissue lysate, LOVO, rat testis tissue, human thyroid gland tissue, human colon cancer tissue, mouse fallopian tissue, PC-3M.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JG38-81

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Bub3 in Lane 1: A549, Lane 2: HL-60, Lane 3: A431, Lane 4: Rat colon . Samples were incubated with Bub3 monoclonal antibody (Product # MA5-34792), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Bub3 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Bub3 monoclonal antibody (Product # MA5-34792), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Bub3 in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Bub3 monoclonal antibody (Product # MA5-34792), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Bub3 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Bub3 monoclonal antibody (Product # MA5-34792), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Bub3 in paraffin-embedded rat testis tissue. Samples were incubated with Bub3 monoclonal antibody (Product # MA5-34792), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Bub3 in paraffin-embedded mouse fallopian tissue. Samples were incubated with Bub3 monoclonal antibody (Product # MA5-34792), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Bub3 in paraffin-embedded human thyroid gland tissue. Samples were incubated with Bub3 monoclonal antibody (Product # MA5-34792), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Bub3 in paraffin-embedded human colon cancer tissue. Samples were incubated with Bub3 monoclonal antibody (Product # MA5-34792), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Bub3 in PC-3M cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with Bub3 monoclonal antibody (Product # MA5-34792) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.