H00005704-M01

antibody from Abnova Corporation

Targeting: PSMC4 MGC13687, MGC23214, MGC8570, MIP224, S6, TBP-7, TBP7

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005704-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005704-M01, RRID:AB_425632

Product name

PSMC4 monoclonal antibody (M01), clone 3G8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant PSMC4.

Antigen sequence

MEEIGILVEKAQDEIPALSVSRPQTGLSFLGPEPE
DLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKK
EFLHAQEEVKRIQSIPLVIGQFLEAVDQNTAIVGS
TTGSNYYVRILSTIDRELLKPNASVALHKHSNALV
DVLPPEADSSIMMLTSDQKPDVMYADIGGMDIQKQ
EVREAVELPLTHFELYKQIGIDPPRGVLMYGPPGC
GKTMLAKAVAHHTTAAFIRVVGSEFVQKYLGEGPR
MVRDVFRLAKENAPAIIFIDEIDAIATKRFDAQTG
ADREVQRILLELLNQMDGFDQNVNVKVIMATNRAD
TLDPALLRPGRLDRKIEFPLPDRRQKRLIFSTITS
KMNLSEEVDLEDYVARPDKISGADINSICQESGML
AVRENRYIVLAKDFEKAYKTVIKKDEQEHEFYK


Isotype

IgG

Antibody clone number

3G8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PSMC4 monoclonal antibody (M01), clone 3G8 Western Blot analysis of PSMC4 expression in HepG2 ( Cat # L019V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PSMC4 expression in transfected 293T cell line by PSMC4 monoclonal antibody (M01), clone 3G8.Lane 1: PSMC4 transfected lysate(47.4 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PSMC4 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to PSMC4 on HepG2 cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to PSMC4 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol