HPA018458

antibody from Atlas Antibodies

Targeting: XIRP1 CMYA1, DKFZp451D042, Xin

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA018458

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA018458, RRID:AB_1858885

Product name

Anti-XIRP1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

RVSTEVAQLKEQTLARLLDIEEAVHKALSSMSSLQ
PEASARGHFQGPPKDHSAHKISVTVSSSARPSGSG
QEVGGQTAVKNQAKVECHTEAQSQVKIRNHT


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human heart muscle and placenta tissues using HPA018458 antibody. Corresponding XIRP1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human colon, heart muscle, liver and testis using Anti-XIRP1 antibody HPA018458 (A) shows similar protein distribution across tissues to independent antibody HPA016750 (B).

Antibody #2 product nr

HPA016750

Antibody provider

Atlas Antibodies

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human heart muscle shows intercalated disc positivity in myocytes.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human heart muscle shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows no positivity in cells in seminiferous ducts.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows weak to moderate cytoplasmic positivity in myocytes.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta shows no positivity in trophoblastic cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human heart muscle shows weak to moderate cytoplasmic positivity in cardiomyocytes.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver using Anti-XIRP1 antibody HPA018458.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon using Anti-XIRP1 antibody HPA018458.

Sample type

HUMAN