Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [31]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB161-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human DC-SIGN/CD209 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human DC-SIGN/CD209 on transfected NIH/3T3 cells and on monocyte derived dendritic cells. Does not react with parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 120507
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Tricarboxylic Acid Cycle Activity and Remodeling of Glycerophosphocholine Lipids Support Cytokine Induction in Response to Fungal Patterns.
Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro.
Clearance of autophagy-associated dying retinal pigment epithelial cells - a possible source for inflammation in age-related macular degeneration.
Metformin Uniquely Prevents Thrombosis by Inhibiting Platelet Activation and mtDNA Release.
HCV RNA Activates APCs via TLR7/TLR8 While Virus Selectively Stimulates Macrophages Without Inducing Antiviral Responses.
Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production.
Live-attenuated measles virus vaccine targets dendritic cells and macrophages in muscle of nonhuman primates.
Porphyromonas gingivalis evasion of autophagy and intracellular killing by human myeloid dendritic cells involves DC-SIGN-TLR2 crosstalk.
Early biodistribution and persistence of a protective live attenuated SIV vaccine elicits localised innate responses in multiple lymphoid tissues.
Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection.
Platelets and erythrocyte-bound platelets bind infectious HIV-1 in plasma of chronically infected patients.
In vivo targeting of human DC-SIGN drastically enhances CD8⁺ T-cell-mediated protective immunity.
CLEC5A is critical for dengue virus-induced inflammasome activation in human macrophages.
Plasmacytoid dendritic cells infiltrate the skin in positive tuberculin skin test indurations.
Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2.
Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite.
In situ distribution of HIV-binding CCR5 and C-type lectin receptors in the human endocervical mucosa.
Semen clusterin is a novel DC-SIGN ligand.
Gram-positive bacteria enhance HIV-1 susceptibility in Langerhans cells, but not in dendritic cells, via Toll-like receptor activation.
Evolving immunosuppressive microenvironment during human cervical carcinogenesis.
Glycosylation-dependent interactions of C-type lectin DC-SIGN with colorectal tumor-associated Lewis glycans impair the function and differentiation of monocyte-derived dendritic cells.
A role of the Ca2+ binding site of DC-SIGN in the phagocytosis of E. coli.
Lewis X oligosaccharides targeting to DC-SIGN enhanced antigen-specific immune response.
Recombinant vesicular stomatitis viruses encoding simian immunodeficiency virus receptors target infected cells and control infection.
Expression of DC-SIGN and DC-SIGNR on human sinusoidal endothelium: a role for capturing hepatitis C virus particles.
BCR/ABL promotes dendritic cell-mediated natural killer cell activation.
NK cell activation by dendritic cells (DCs) requires the formation of a synapse leading to IL-12 polarization in DCs.
Urine dendritic cells: a noninvasive probe for immune activity in bladder cancer?
Dendritic cells in the human decidua.
DC-SIGN is the major Mycobacterium tuberculosis receptor on human dendritic cells.
Rhesus macaque dendritic cells efficiently transmit primate lentiviruses independently of DC-SIGN.
Márquez S, Fernández JJ, Mancebo C, Herrero-Sánchez C, Alonso S, Sandoval TA, Rodríguez Prados M, Cubillos-Ruiz JR, Montero O, Fernández N, Sánchez Crespo M
Cell reports 2019 Apr 9;27(2):525-536.e4
Cell reports 2019 Apr 9;27(2):525-536.e4
Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro.
Smith IM, Baker A, Christensen JE, Boekhout T, Frøkiær H, Arneborg N, Jespersen L
PloS one 2016;11(11):e0167410
PloS one 2016;11(11):e0167410
Clearance of autophagy-associated dying retinal pigment epithelial cells - a possible source for inflammation in age-related macular degeneration.
Szatmári-Tóth M, Kristóf E, Veréb Z, Akhtar S, Facskó A, Fésüs L, Kauppinen A, Kaarniranta K, Petrovski G
Cell death & disease 2016 Sep 8;7(9):e2367
Cell death & disease 2016 Sep 8;7(9):e2367
Metformin Uniquely Prevents Thrombosis by Inhibiting Platelet Activation and mtDNA Release.
Xin G, Wei Z, Ji C, Zheng H, Gu J, Ma L, Huang W, Morris-Natschke SL, Yeh JL, Zhang R, Qin C, Wen L, Xing Z, Cao Y, Xia Q, Lu Y, Li K, Niu H, Lee KH, Huang W
Scientific reports 2016 Nov 2;6:36222
Scientific reports 2016 Nov 2;6:36222
HCV RNA Activates APCs via TLR7/TLR8 While Virus Selectively Stimulates Macrophages Without Inducing Antiviral Responses.
Zhang Y, El-Far M, Dupuy FP, Abdel-Hakeem MS, He Z, Procopio FA, Shi Y, Haddad EK, Ancuta P, Sekaly RP, Said EA
Scientific reports 2016 Jul 7;6:29447
Scientific reports 2016 Jul 7;6:29447
Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production.
Fernandes RK, Bachiega TF, Rodrigues DR, Golim Mde A, Dias-Melicio LA, Balderramas Hde A, Kaneno R, Soares ÂM
PloS one 2015;10(3):e0120948
PloS one 2015;10(3):e0120948
Live-attenuated measles virus vaccine targets dendritic cells and macrophages in muscle of nonhuman primates.
Rennick LJ, de Vries RD, Carsillo TJ, Lemon K, van Amerongen G, Ludlow M, Nguyen DT, Yüksel S, Verburgh RJ, Haddock P, McQuaid S, Duprex WP, de Swart RL
Journal of virology 2015 Feb;89(4):2192-200
Journal of virology 2015 Feb;89(4):2192-200
Porphyromonas gingivalis evasion of autophagy and intracellular killing by human myeloid dendritic cells involves DC-SIGN-TLR2 crosstalk.
El-Awady AR, Miles B, Scisci E, Kurago ZB, Palani CD, Arce RM, Waller JL, Genco CA, Slocum C, Manning M, Schoenlein PV, Cutler CW
PLoS pathogens 2015 Feb;10(2):e1004647
PLoS pathogens 2015 Feb;10(2):e1004647
Early biodistribution and persistence of a protective live attenuated SIV vaccine elicits localised innate responses in multiple lymphoid tissues.
Ferguson D, Mattiuzzo G, Ham C, Stebbings R, Li B, Rose NJ, Mee ET, Smith D, Page M, Cranage MP, Almond N, Towers GJ, Berry NJ
PloS one 2014;9(8):e104390
PloS one 2014;9(8):e104390
Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection.
Cerny D, Haniffa M, Shin A, Bigliardi P, Tan BK, Lee B, Poidinger M, Tan EY, Ginhoux F, Fink K
PLoS pathogens 2014 Dec;10(12):e1004548
PLoS pathogens 2014 Dec;10(12):e1004548
Platelets and erythrocyte-bound platelets bind infectious HIV-1 in plasma of chronically infected patients.
Beck Z, Jagodzinski LL, Eller MA, Thelian D, Matyas GR, Kunz AN, Alving CR
PloS one 2013;8(11):e81002
PloS one 2013;8(11):e81002
In vivo targeting of human DC-SIGN drastically enhances CD8⁺ T-cell-mediated protective immunity.
Hesse C, Ginter W, Förg T, Mayer CT, Baru AM, Arnold-Schrauf C, Unger WW, Kalay H, van Kooyk Y, Berod L, Sparwasser T
European journal of immunology 2013 Oct;43(10):2543-53
European journal of immunology 2013 Oct;43(10):2543-53
CLEC5A is critical for dengue virus-induced inflammasome activation in human macrophages.
Wu MF, Chen ST, Yang AH, Lin WW, Lin YL, Chen NJ, Tsai IS, Li L, Hsieh SL
Blood 2013 Jan 3;121(1):95-106
Blood 2013 Jan 3;121(1):95-106
Plasmacytoid dendritic cells infiltrate the skin in positive tuberculin skin test indurations.
Bond E, Liang F, Sandgren KJ, Smed-Sörensen A, Bergman P, Brighenti S, Adams WC, Betemariam SA, Rangaka MX, Lange C, Wilkinson RJ, Andersson J, Loré K
The Journal of investigative dermatology 2012 Jan;132(1):114-23
The Journal of investigative dermatology 2012 Jan;132(1):114-23
Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2.
Johnson TR, McLellan JS, Graham BS
Journal of virology 2012 Feb;86(3):1339-47
Journal of virology 2012 Feb;86(3):1339-47
Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite.
Emara M, Royer PJ, Mahdavi J, Shakib F, Ghaemmaghami AM
The Journal of biological chemistry 2012 Feb 17;287(8):5756-63
The Journal of biological chemistry 2012 Feb 17;287(8):5756-63
In situ distribution of HIV-binding CCR5 and C-type lectin receptors in the human endocervical mucosa.
Hirbod T, Kaldensjö T, Broliden K
PloS one 2011;6(9):e25551
PloS one 2011;6(9):e25551
Semen clusterin is a novel DC-SIGN ligand.
Sabatte J, Faigle W, Ceballos A, Morelle W, Rodríguez Rodrígues C, Remes Lenicov F, Thépaut M, Fieschi F, Malchiodi E, Fernández M, Arenzana-Seisdedos F, Lortat-Jacob H, Michalski JC, Geffner J, Amigorena S
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 15;187(10):5299-309
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 15;187(10):5299-309
Gram-positive bacteria enhance HIV-1 susceptibility in Langerhans cells, but not in dendritic cells, via Toll-like receptor activation.
Ogawa Y, Kawamura T, Kimura T, Ito M, Blauvelt A, Shimada S
Blood 2009 May 21;113(21):5157-66
Blood 2009 May 21;113(21):5157-66
Evolving immunosuppressive microenvironment during human cervical carcinogenesis.
Kobayashi A, Weinberg V, Darragh T, Smith-McCune K
Mucosal immunology 2008 Sep;1(5):412-20
Mucosal immunology 2008 Sep;1(5):412-20
Glycosylation-dependent interactions of C-type lectin DC-SIGN with colorectal tumor-associated Lewis glycans impair the function and differentiation of monocyte-derived dendritic cells.
Nonaka M, Ma BY, Murai R, Nakamura N, Baba M, Kawasaki N, Hodohara K, Asano S, Kawasaki T
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3347-56
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3347-56
A role of the Ca2+ binding site of DC-SIGN in the phagocytosis of E. coli.
Iyori M, Ohtani M, Hasebe A, Totsuka Y, Shibata KI
Biochemical and biophysical research communications 2008 Dec 12;377(2):367-372
Biochemical and biophysical research communications 2008 Dec 12;377(2):367-372
Lewis X oligosaccharides targeting to DC-SIGN enhanced antigen-specific immune response.
Wang J, Zhang Y, Wei J, Zhang X, Zhang B, Zhu Z, Zou W, Wang Y, Mou Z, Ni B, Wu Y
Immunology 2007 Jun;121(2):174-82
Immunology 2007 Jun;121(2):174-82
Recombinant vesicular stomatitis viruses encoding simian immunodeficiency virus receptors target infected cells and control infection.
Okuma K, Boritz E, Walker J, Sarkar A, Alexander L, Rose JK
Virology 2006 Mar 1;346(1):86-97
Virology 2006 Mar 1;346(1):86-97
Expression of DC-SIGN and DC-SIGNR on human sinusoidal endothelium: a role for capturing hepatitis C virus particles.
Lai WK, Sun PJ, Zhang J, Jennings A, Lalor PF, Hubscher S, McKeating JA, Adams DH
The American journal of pathology 2006 Jul;169(1):200-8
The American journal of pathology 2006 Jul;169(1):200-8
BCR/ABL promotes dendritic cell-mediated natural killer cell activation.
Terme M, Borg C, Guilhot F, Masurier C, Flament C, Wagner EF, Caillat-Zucman S, Bernheim A, Turhan AG, Caignard A, Zitvogel L
Cancer research 2005 Jul 15;65(14):6409-17
Cancer research 2005 Jul 15;65(14):6409-17
NK cell activation by dendritic cells (DCs) requires the formation of a synapse leading to IL-12 polarization in DCs.
Borg C, Jalil A, Laderach D, Maruyama K, Wakasugi H, Charrier S, Ryffel B, Cambi A, Figdor C, Vainchenker W, Galy A, Caignard A, Zitvogel L
Blood 2004 Nov 15;104(10):3267-75
Blood 2004 Nov 15;104(10):3267-75
Urine dendritic cells: a noninvasive probe for immune activity in bladder cancer?
Beatty JD, Islam S, North ME, Knight SC, Ogden CW
BJU international 2004 Dec;94(9):1377-83
BJU international 2004 Dec;94(9):1377-83
Dendritic cells in the human decidua.
Gardner L, Moffett A
Biology of reproduction 2003 Oct;69(4):1438-46
Biology of reproduction 2003 Oct;69(4):1438-46
DC-SIGN is the major Mycobacterium tuberculosis receptor on human dendritic cells.
Tailleux L, Schwartz O, Herrmann JL, Pivert E, Jackson M, Amara A, Legres L, Dreher D, Nicod LP, Gluckman JC, Lagrange PH, Gicquel B, Neyrolles O
The Journal of experimental medicine 2003 Jan 6;197(1):121-7
The Journal of experimental medicine 2003 Jan 6;197(1):121-7
Rhesus macaque dendritic cells efficiently transmit primate lentiviruses independently of DC-SIGN.
Wu L, Bashirova AA, Martin TD, Villamide L, Mehlhop E, Chertov AO, Unutmaz D, Pope M, Carrington M, KewalRamani VN
Proceedings of the National Academy of Sciences of the United States of America 2002 Feb 5;99(3):1568-73
Proceedings of the National Academy of Sciences of the United States of America 2002 Feb 5;99(3):1568-73
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- DC-SIGN in Human Dendritic Cells. DC-SIGN was detected in immersion fixed mature human dendritic cells using Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of DC-SIGN in Human Monocyte Derived Dendritic Cells by Flow Cytometry. Human monocyte derived dendritic cells were stained with Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161) followed by PE-conjugated anti-mouse IgG (Catalog # F0102B) and Anti-Human B7-2/CD86 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB141F). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of DC-SIGN in Human DC-SIGN Transfected 3T3 Mouse Cell Line by Flow Cytometry. Human DC-SIGN and DC-SIGN2 transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161, filled histograms) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
