PAB20228

antibody from Abnova Corporation

Targeting: ANKLE2 KIAA0692, Lem4, LEMD7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20228

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20228, RRID:AB_10963269

Product name

ANKLE2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ANKLE2.

Antigen sequence

SETVNKERANSYKNPRTQDLTAKLRKAVEKGEEDT
FSDLIWSNPRYLIGSGDNPTIVQEGCRYNVMHVAA
KENQASICQLTLDVLENPDFMRLMYPDDDEAMLQK
RIRYVVDLYLNTPDKMGYDTPLHFACKFGNADVVN
VLSSH


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with ANKLE2 polyclonal antibody (Cat # PAB20228).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with ANKLE2 polyclonal antibody (Cat # PAB20228) at 1-4 ug/mL dilution shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with ANKLE2 polyclonal antibody (Cat # PAB20228) shows cytoplasmic and nuclear positivity in cells in seminiferus ducts at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)