FNab09185

antibody from FineTest Biotech

Targeting: UBE2T FANCT, HSPC150

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

FNab09185

Provider

FineTest Biotech

Product name

UBE2T/HSPC150 antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

The ubiquitin(Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic(UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs(TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

ubiquitin-conjugating enzyme E2T(pu
tative)


Epitope

IgG

Isotype

IgG

Vial size

100¦Ìg

Concentration

Immunogen affinity purified

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20¡æ for 12 months(Avoid repeated freeze / thaw cycles.)

Handling

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20¡æ for 12 months(Avoid repeated freeze / thaw cycles.)