Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB10553 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10553, RRID:AB_11190135
- Product name
- CHD3 monoclonal antibody, clone 2G4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant CHD3.
- Isotype
- IgG
- Antibody clone number
- 2G4
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using CHD3 monoclonal antibody, clone 2G4 (Cat # MAB10553) against HeLa (1), K-562 (2), Jurkat (3), NTERA-2 (4), HEK293 (5), Raji (6) cell lysate and mouse brain (7) tissue lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using CHD3 monoclonal antibody, clone 2G4 (Cat # MAB10553) (green). Blue: DRAQ5 fluorescent DNA dye.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using CHD3 monoclonal antibody, clone 2G4 (Cat # MAB10553) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of K-562 cells using CHD3 monoclonal antibody, clone 2G4 (Cat # MAB10553) (green) and negative control (purple).
- Validation comment
- Flow Cytometry