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- Antibody Data
- Antigen structure
- References [22]
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- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- ABIN152993 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-TPX2, Microtubule-Associated, Homolog (Xenopus Laevis) (TPX2) (C-Term) antibody
- Antibody type
- Polyclonal
- Description
- Unpurified
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Rabbit
- Epitope
- C-Term
- Vial size
- 0.1 mL
- Storage
- Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
- Handling
- Avoid freeze-thaw cycles
Submitted references HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development.
Regulation of Kif15 localization and motility by the C-terminus of TPX2 and microtubule dynamics.
FSH Regulates mRNA Translation in Mouse Oocytes and Promotes Developmental Competence.
The Clathrin-dependent Spindle Proteome.
A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly.
The selective inhibition of protein phosphatase-1 results in mitotic catastrophe and impaired tumor growth.
Histone deacetylase inhibitors promote glioma cell death by G2 checkpoint abrogation leading to mitotic catastrophe.
Enhanced protein degradation by branched ubiquitin chains.
Microtubule-dependent regulation of mitotic protein degradation.
The cytoskeletal protein RHAMM and ERK1/2 activity maintain the pluripotency of murine embryonic stem cells.
NQO1 prevents radiation-induced aneuploidy by interacting with Aurora-A.
Aurora A kinase regulates mammary epithelial cell fate by determining mitotic spindle orientation in a Notch-dependent manner.
Chromosomal gain promotes formation of a steep RanGTP gradient that drives mitosis in aneuploid cells.
Somatic cells regulate maternal mRNA translation and developmental competence of mouse oocytes.
Importin-β negatively regulates multiple aspects of mitosis including RANGAP1 recruitment to kinetochores.
Poleward transport of TPX2 in the mammalian mitotic spindle requires dynein, Eg5, and microtubule flux.
Identification of a physiological E2 module for the human anaphase-promoting complex.
A survivin-ran complex regulates spindle formation in tumor cells.
Tumor cell dependence on Ran-GTP-directed mitosis.
Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A.
hTPX2 is required for normal spindle morphology and centrosome integrity during vertebrate cell division.
p100: a novel proliferation-associated nuclear protein specifically restricted to cell cycle phases S, G2, and M.
Connell M, Chen H, Jiang J, Kuan CW, Fotovati A, Chu TL, He Z, Lengyell TC, Li H, Kroll T, Li AM, Goldowitz D, Frappart L, Ploubidou A, Patel MS, Pilarski LM, Simpson EM, Lange PF, Allan DW, Maxwell CA
eLife 2017 Oct 10;6
eLife 2017 Oct 10;6
Regulation of Kif15 localization and motility by the C-terminus of TPX2 and microtubule dynamics.
Mann BJ, Balchand SK, Wadsworth P
Molecular biology of the cell 2017 Jan 1;28(1):65-75
Molecular biology of the cell 2017 Jan 1;28(1):65-75
FSH Regulates mRNA Translation in Mouse Oocytes and Promotes Developmental Competence.
Franciosi F, Manandhar S, Conti M
Endocrinology 2016 Feb;157(2):872-82
Endocrinology 2016 Feb;157(2):872-82
The Clathrin-dependent Spindle Proteome.
Rao SR, Flores-Rodriguez N, Page SL, Wong C, Robinson PJ, Chircop M
Molecular & cellular proteomics : MCP 2016 Aug;15(8):2537-53
Molecular & cellular proteomics : MCP 2016 Aug;15(8):2537-53
A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly.
Barr AR, Bakal C
Scientific reports 2015 Jun 3;5:10564
Scientific reports 2015 Jun 3;5:10564
The selective inhibition of protein phosphatase-1 results in mitotic catastrophe and impaired tumor growth.
Winkler C, De Munter S, Van Dessel N, Lesage B, Heroes E, Boens S, Beullens M, Van Eynde A, Bollen M
Journal of cell science 2015 Dec 15;128(24):4526-37
Journal of cell science 2015 Dec 15;128(24):4526-37
Histone deacetylase inhibitors promote glioma cell death by G2 checkpoint abrogation leading to mitotic catastrophe.
Cornago M, Garcia-Alberich C, Blasco-Angulo N, Vall-Llaura N, Nager M, Herreros J, Comella JX, Sanchis D, Llovera M
Cell death & disease 2014 Oct 2;5:e1435
Cell death & disease 2014 Oct 2;5:e1435
Enhanced protein degradation by branched ubiquitin chains.
Meyer HJ, Rape M
Cell 2014 May 8;157(4):910-21
Cell 2014 May 8;157(4):910-21
Microtubule-dependent regulation of mitotic protein degradation.
Song L, Craney A, Rape M
Molecular cell 2014 Jan 23;53(2):179-92
Molecular cell 2014 Jan 23;53(2):179-92
The cytoskeletal protein RHAMM and ERK1/2 activity maintain the pluripotency of murine embryonic stem cells.
Jiang J, Mohan P, Maxwell CA
PloS one 2013;8(9):e73548
PloS one 2013;8(9):e73548
NQO1 prevents radiation-induced aneuploidy by interacting with Aurora-A.
Park MT, Oh ET, Song MJ, Lee H, Choi EK, Park HJ
Carcinogenesis 2013 Nov;34(11):2470-85
Carcinogenesis 2013 Nov;34(11):2470-85
Aurora A kinase regulates mammary epithelial cell fate by determining mitotic spindle orientation in a Notch-dependent manner.
Regan JL, Sourisseau T, Soady K, Kendrick H, McCarthy A, Tang C, Brennan K, Linardopoulos S, White DE, Smalley MJ
Cell reports 2013 Jul 11;4(1):110-23
Cell reports 2013 Jul 11;4(1):110-23
Chromosomal gain promotes formation of a steep RanGTP gradient that drives mitosis in aneuploid cells.
Hasegawa K, Ryu SJ, Kaláb P
The Journal of cell biology 2013 Jan 21;200(2):151-61
The Journal of cell biology 2013 Jan 21;200(2):151-61
Somatic cells regulate maternal mRNA translation and developmental competence of mouse oocytes.
Chen J, Torcia S, Xie F, Lin CJ, Cakmak H, Franciosi F, Horner K, Onodera C, Song JS, Cedars MI, Ramalho-Santos M, Conti M
Nature cell biology 2013 Dec;15(12):1415-23
Nature cell biology 2013 Dec;15(12):1415-23
Importin-β negatively regulates multiple aspects of mitosis including RANGAP1 recruitment to kinetochores.
Roscioli E, Di Francesco L, Bolognesi A, Giubettini M, Orlando S, Harel A, Schininà ME, Lavia P
The Journal of cell biology 2012 Feb 20;196(4):435-50
The Journal of cell biology 2012 Feb 20;196(4):435-50
Poleward transport of TPX2 in the mammalian mitotic spindle requires dynein, Eg5, and microtubule flux.
Ma N, Tulu US, Ferenz NP, Fagerstrom C, Wilde A, Wadsworth P
Molecular biology of the cell 2010 Mar 15;21(6):979-88
Molecular biology of the cell 2010 Mar 15;21(6):979-88
Identification of a physiological E2 module for the human anaphase-promoting complex.
Williamson A, Wickliffe KE, Mellone BG, Song L, Karpen GH, Rape M
Proceedings of the National Academy of Sciences of the United States of America 2009 Oct 27;106(43):18213-8
Proceedings of the National Academy of Sciences of the United States of America 2009 Oct 27;106(43):18213-8
A survivin-ran complex regulates spindle formation in tumor cells.
Xia F, Canovas PM, Guadagno TM, Altieri DC
Molecular and cellular biology 2008 Sep;28(17):5299-311
Molecular and cellular biology 2008 Sep;28(17):5299-311
Tumor cell dependence on Ran-GTP-directed mitosis.
Xia F, Lee CW, Altieri DC
Cancer research 2008 Mar 15;68(6):1826-33
Cancer research 2008 Mar 15;68(6):1826-33
Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A.
Bird AW, Hyman AA
The Journal of cell biology 2008 Jul 28;182(2):289-300
The Journal of cell biology 2008 Jul 28;182(2):289-300
hTPX2 is required for normal spindle morphology and centrosome integrity during vertebrate cell division.
Garrett S, Auer K, Compton DA, Kapoor TM
Current biology : CB 2002 Dec 10;12(23):2055-9
Current biology : CB 2002 Dec 10;12(23):2055-9
p100: a novel proliferation-associated nuclear protein specifically restricted to cell cycle phases S, G2, and M.
Heidebrecht HJ, Buck F, Steinmann J, Sprenger R, Wacker HH, Parwaresch R
Blood 1997 Jul 1;90(1):226-33
Blood 1997 Jul 1;90(1):226-33
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- WB
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Staining of HeLa cells fixed in 3.5% paraformaldehyde using ABIN152993 (1:1,000). Nuclear staining during interphase and spindle staining during mitosis.
