PA3-113

Jayawardena D, Guzman G, Gill RK, Alrefai WA, Onyuksel H, Dudeja PK
American journal of physiology. Gastrointestinal and liver physiology 2017 Jul 1;313(1):G16-G25

Atypical nuclear localization of VIP receptors in glioma cell lines and patients.

Barbarin A, Séité P, Godet J, Bensalma S, Muller JM, Chadéneau C
Biochemical and biophysical research communications 2014 Nov 28;454(4):524-30

The vasoactive intestinal peptide-receptor system is involved in human glioblastoma cell migration.

Cochaud S, Chevrier L, Meunier AC, Brillet T, Chadéneau C, Muller JM
Neuropeptides 2010 Oct;44(5):373-83

Vasoactive intestinal peptide (VIP) induces malignant transformation of the human prostate epithelial cell line RWPE-1.

Fernández-Martínez AB, Bajo AM, Isabel Arenas M, Sánchez-Chapado M, Prieto JC, Carmena MJ
Cancer letters 2010 Dec 18;299(1):11-21

Immunocytochemical identification of VPAC1, VPAC2, and PAC1 receptors in normal and neoplastic human tissues with subtype-specific antibodies.

Schulz S, Röcken C, Mawrin C, Weise W, Höllt V, Schulz S
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Dec 15;10(24):8235-42

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on membrane enriched cell extracts (30 µg lysate) of HT-29 (Lane 1), COLO 205 (Lane 2), Caco-2 (Lane 3), MCF7 (Lane 4), MDA-MB-231 (Lane 5), T-47D (Lane 6), PC-3 (Lane 7) and LNCaP (Lane 8). The blots were probed with Anti- VIPR1 Rabbit Polyclonal Antibody (Product # PA3-113, 1:250 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4µg/mL, 1:2500 dilution). A 55 kDa band corresponding to VIPR1 was observed across all cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of VPAC1 Receptor in human intestine samples using Product # PA3-113.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 10 Immunohistochemistry of 12 selected genes expression in two lung adenocarcinoma cases. (A-L) Representative pictures of an IHC staining with paraffin-embedded tissue sections demonstrate the selected genes' protein expression patterns (brown signal) in adjacent tissue (left panel) and matched malignant tumor tissue (right panel). The 12 selected genes were in order as follows: ADIPOR1, ARRB1, S100A12, CD1b, HAMP, HMOX1, KL, S100A7, S100A2, VEGFA, VIPR1, and TUBB3.