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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- GTX48495 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48495, RRID:AB_11176182
- Product name
- POLK antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Goat
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Western blot using GeneTex's Affinity Purified anti-POLK antibody shows detection of a band ~98 kDa corresponding to human POLK (arrowhead). Approximately 35 ?g of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4¢X followed by washes and reaction with a 1:20,000 dilution of IRDye800 conjugated rabbit anti-Goat IgG [H&L] MXHu for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey? Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
